Southwest Landscape Architecture Engineering Research Center of State Forestry Administration, Landscape Architecture and Horticulture Science School, Southwest Forestry University, Kunming 650000, China.
Genes (Basel). 2022 Oct 31;13(11):1993. doi: 10.3390/genes13111993.
Scopoletin, the main component of clinical drugs and the functional component of health products, is highly abundant in noni fruit (). Multiple enzyme genes regulate scopoletin accumulation. In the present study, differentially expressed genes of noni were analyzed by RNA sequencing (RNA-Seq) and the full-length genes by isoform-sequencing (Iso-Seq) to find the critical genes in the scopoletin accumulation mechanism pathway. A total of 32,682 full-length nonchimeric reads (FLNC) were obtained, out of which 16,620 non-redundant transcripts were validated. Based on KEGG (Kyoto Encyclopedia of Genes and Genomes) annotation and differential expression analysis, two differentially expressed genes, caffeic acid 3-O-methyltransferase () and caffeoyl-CoA O-methyltransferase (), were found in the scopoletin accumulation pathway of noni. Real-time quantitative polymerase chain reaction (q-PCR), phylogenetic tree analysis, gene expression analysis, and the change in scopoletin content confirmed that these two proteins are important in this pathway. Based on these results, the current study supposed that and play a significant role in the accumulation of scopoletin in noni fruit, and (gene number: gene 7446, gene 8422, and gene 6794) and (gene number: gene 12,084) were more significant. These results provide the importance of and and a basis for further understanding the accumulation mechanism of scopoletin in noni.
松脂素是临床药物的主要成分和保健品的功能成分，在诺丽果中含量丰富（）。多种酶基因调控松脂素的积累。本研究通过 RNA 测序（RNA-Seq）和异构体测序（Iso-Seq）分析诺丽果的差异表达基因，找到松脂素积累机制途径中的关键基因。共获得 32682 条全长非嵌合读数（FLNC），其中 16620 个非冗余转录本得到验证。基于 KEGG（京都基因与基因组百科全书）注释和差异表达分析，在诺丽果的松脂素积累途径中发现了两个差异表达基因，咖啡酸 3-O-甲基转移酶（）和咖啡酰辅酶 A O-甲基转移酶（）。实时定量聚合酶链反应（q-PCR）、系统发育树分析、基因表达分析和松脂素含量变化证实，这两种蛋白质在该途径中很重要。基于这些结果，本研究假设和在诺丽果果实中松脂素的积累中起重要作用，和（基因编号：基因 7446、基因 8422 和基因 6794）和（基因编号：基因 12、084）更为显著。这些结果为进一步了解诺丽果中松脂素的积累机制提供了和的重要性和依据。
