Mass Spectrometry for Biology Unit, Institut Pasteur, CNRS USR 2000, Paris, France.
Bioinformatics and Biostatistics HUB, Computational Biology Department, Institut Pasteur, CNRS USR 3756, Paris, France.
Bioinformatics. 2021 Aug 9;37(15):2206-2208. doi: 10.1093/bioinformatics/btaa958.
We present a high-performance software integrating shotgun with top-down proteomic data. The tool can deal with multiple experiments and search engines. Enable rapid and easy visualization, manual validation and comparison of the identified proteoform sequences including the post-translational modification characterization.
We demonstrate the effectiveness of our approach on a large-scale Escherichia coli dataset; ProteoCombiner unambiguously shortlisted proteoforms among those identified by the multiple search engines.
ProteoCombiner, a demonstration video and user tutorial are freely available at https://proteocombiner.pasteur.fr, for academic use; all data are thus available from the ProteomeXchange consortium (identifier PXD017618).
Supplementary data are available at Bioinformatics online.
我们提出了一种高性能的软件，将鸟枪法与自上而下的蛋白质组学数据相结合。该工具可以处理多个实验和搜索引擎。能够快速、轻松地可视化、手动验证和比较鉴定的肽段序列，包括对翻译后修饰的特征描述。
我们在一个大规模的大肠杆菌数据集上证明了我们方法的有效性；ProteoCombiner 能够在多个搜索引擎鉴定的肽段中明确地进行短名单筛选。
ProteoCombiner、演示视频和用户教程可在 https://proteocombiner.pasteur.fr 上免费获取，供学术使用；所有数据均可从 ProteomeXchange 联盟（标识符 PXD017618）获取。
补充数据可在 Bioinformatics 在线获取。
