Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
Methods Mol Biol. 2021;2185:159-179. doi: 10.1007/978-1-0716-0810-4_10.
Leukemic stem cells are highly dynamic and heterogeneous. Analysis of leukemic stem cells at the single-cell level should provide a wealth of insights that would not be possible using bulk measurements. Mass spectrometry (MS)-based proteomic workflows can quantify hundreds or thousands of proteins from a biological sample and has proven invaluable for biomedical research, but samples comprising large numbers of cells are typically required due to limited sensitivity. Recent developments in sample processing, chromatographic separations, and MS instrumentation are now extending in-depth proteome profiling to single mammalian cells. Here, we describe specific techniques that increase the sensitivity of single-cell proteomics by orders of magnitude, enabling the promise of single-cell proteomics to become a reality. We anticipate such techniques can significantly advance the understanding of leukemic stem cells.
白血病干细胞具有高度的动态性和异质性。在单细胞水平上分析白血病干细胞应该能够提供大量的信息，而这些信息是使用批量测量无法获得的。基于质谱（MS）的蛋白质组学工作流程可以从生物样本中定量数百或数千种蛋白质，并且已被证明对生物医学研究非常有价值，但由于灵敏度有限，通常需要包含大量细胞的样本。最近在样品处理、色谱分离和 MS 仪器方面的发展，现在正在将深度蛋白质组分析扩展到单个哺乳动物细胞。在这里，我们描述了通过数量级提高单细胞蛋白质组学灵敏度的特定技术，使单细胞蛋白质组学的承诺成为现实。我们预计这些技术可以极大地促进对白血病干细胞的理解。
