Department of Regulatory Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, Telangana, India.
Department of Botany, Rajiv Gandhi University, Ron Hills, Doimukh, Arunachal Pradesh, India.
Environ Toxicol. 2021 Apr;36(4):530-539. doi: 10.1002/tox.23058. Epub 2020 Nov 9.
Borneol is a commonly used flavouring substance in traditional Chinese medicine, which possesses several pharmacological activities including analgesic, antiinflammatory, and antioxidant properties. The aim of this study was to investigate the effects of borneol on cerulein-induced acute pancreatitis (AP) model. Swiss albino mice were pretreated with borneol (100 and 300 mg/kg) daily for 7 days, before six consecutive injections of cerulein (50 μg/kg/hr, intraperitoneally). The protective effect of borneol was studied by biochemical, enzyme linked immunosorbent assay, histological, immunoblotting, and immunohistochemical analysis. Oral administration of borneol significantly attenuated pancreatic damage by reducing amylase, lipase levels and histological changes. Borneol attenuated cerulein-induced oxidative-nitrosative stress by decreasing malondialdehyde, nitrite levels, and elevating reduced glutathione levels. Pancreatic inflammation was ameliorated by inhibiting myeloperoxidase activity and pro-inflammatory cytokine (Interleukins and TNF-α) levels. Furthermore, borneol administration significantly increased nuclear factor E2-related factor 2 (Nrf2), superoxide dismutase (SOD1) expression and reduced phospho-NF-κB p65 expression. Treatment with borneol significantly inhibited TNF-α, IL-1β, IL-6, and inducible nitric oxide synthase expression in cerulein-induced AP mouse model. Together, these results indicate that borneol which is currently used as US-FDA approved food adjuvant has the potential to attenuate cerulein-induced AP possibly by reducing the oxidative damage and pancreatic inflammation by modulating Nrf2/NF-κB pathway.
龙脑是一种常用的中药调味物质，具有多种药理活性，包括镇痛、抗炎和抗氧化作用。本研究旨在探讨龙脑对鹅膏蕈碱诱导的急性胰腺炎（AP）模型的影响。瑞士白化小鼠用龙脑（100 和 300mg/kg）预处理，每天一次，共 7 天，然后连续 6 次腹腔内注射鹅膏蕈碱（50μg/kg/hr）。通过生化、酶联免疫吸附试验、组织学、免疫印迹和免疫组织化学分析研究龙脑的保护作用。龙脑的口服给药通过降低淀粉酶、脂肪酶水平和组织学变化显著减轻胰腺损伤。龙脑通过降低丙二醛、亚硝酸盐水平和提高还原型谷胱甘肽水平来减轻鹅膏蕈碱诱导的氧化-硝化应激。通过抑制髓过氧化物酶活性和促炎细胞因子（白细胞介素和 TNF-α）水平来改善胰腺炎症。此外，龙脑给药显著增加核因子 E2 相关因子 2（Nrf2）、超氧化物歧化酶（SOD1）的表达，并降低磷酸化 NF-κB p65 的表达。龙脑治疗显著抑制鹅膏蕈碱诱导的 AP 小鼠模型中 TNF-α、IL-1β、IL-6 和诱导型一氧化氮合酶的表达。总之，这些结果表明，龙脑作为美国 FDA 批准的食品添加剂，具有通过调节 Nrf2/NF-κB 通路减轻鹅膏蕈碱诱导的 AP 的潜力，可能是通过减少氧化损伤和胰腺炎症。
