Enhanced Production of Ethyl Lactate in by Genetic Modification.

Ethyl lactate is an important flavor substance in baijiu, and it is also one of the common raw materials in the production of flavors and spices. In this study, we first established the ethyl lactate biosynthesis pathway in α(L) by introducing propionyl coenzyme A transferase () and alcohol acyltransferase (), and the results showed that strain α(L)-CP-Ae produced the most ethyl lactate 239.53 ± 5.45 mg/L. Subsequently, the copy number of the gene and gene was increased, and the modified strain α(L)-tCP-tAe produced 346.39 ± 3.99 mg/L ethyl lactate. Finally, the porin gene () and the mitochondrial pyruvate carrier gene () were knocked to impede mitochondrial transport of pyruvate, and the final modified strain α(L)-tCP-tAeΔpor produced ethyl lactate 420.48 ± 6.03 mg/L.