环扎缝线诱导的小鼠模型中眼球表面静脉压升高。

Increased episcleral venous pressure in a mouse model of circumlimbal suture induced ocular hypertension.

机构信息

Department of Optometry and Vision Sciences, University of Melbourne, Parkville, Victoria, Australia.

North Texas Eye Research Institute, Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas, USA.

出版信息

Exp Eye Res. 2021 Jan;202:108348. doi: 10.1016/j.exer.2020.108348. Epub 2020 Nov 6.

DOI:10.1016/j.exer.2020.108348

PMID:33166503

Abstract

PURPOSE

To investigate changes in aqueous humor dynamics during intraocular pressure (IOP) elevation induced by circumlimbal suture in mice.

METHODS

Ocular hypertension (OHT) was induced by applying a circumlimbal suture behind the limbus in male adult C57BL6/J mice. In the OHT group, the suture was left in place for an average of 8 weeks (n = 10, OHT group). In the sham control group the suture was cut at 2 days (n = 9, sham group) and in the naïve control group (n = 5) no suture was implanted. IOP was measured at baseline across 3 days, 1 h post-suture implantation, and at the chronic endpoint. Anterior segments were assessed using optical coherence tomography (OCT). Episcleral venous pressure (EVP), total outflow facility (C), uveoscleral outflow (Fu) and aqueous humor flow rate (Fin) were determined using a constant-flow infusion model.

RESULTS

All aqueous dynamic and chronic IOP outcome measures showed no difference between sham and naïve controls (p > 0.05) and thus these groups were combined into a single control group. IOP was elevated in OHT group compared with controls (p < 0.01). Chronic suture implantation did not change pupil size, anterior chamber depth or iridocorneal angles (p > 0.05). EVP was significantly higher in OHT eyes compared to control eyes (p < 0.01). There was no statistical difference in C, Fu and Fin between groups (p > 0.05). A significant linear correlation was found between IOP and EVP (R = 0.35, p = 0.001).

CONCLUSIONS

Circumlimbal suture implantation in mouse eyes results in chronic IOP elevation without angle closure. Chronic IOP elevation is likely to reflect higher EVP.

摘要

目的

研究环扎缝线引起的眼内压（IOP）升高过程中房水动力学的变化。

方法

在雄性成年 C57BL6/J 小鼠的角膜缘后进行环扎缝线以诱导高眼压（OHT）。在 OHT 组中，缝线平均放置 8 周（n=10，OHT 组）。在假手术对照组中，缝线在 2 天（n=9，假手术组）时剪断，在未处理对照组（n=5）中则不植入缝线。在 3 天内、缝线植入后 1 小时和慢性终点测量基础 IOP。使用光学相干断层扫描（OCT）评估眼前段。使用恒流灌注模型测量巩膜静脉压（EVP）、总流出阻力（C）、葡萄膜巩膜流出（Fu）和房水流出率（Fin）。

结果

所有房水动力学和慢性 IOP 结果测量值在假手术和未处理对照组之间均无差异（p>0.05），因此将这两组合并为一个对照组。与对照组相比，OHT 组的 IOP 升高（p<0.01）。慢性缝线植入未改变瞳孔大小、前房深度或虹膜角膜角（p>0.05）。与对照组相比，OHT 眼的 EVP 显著升高（p<0.01）。各组之间的 C、Fu 和 Fin 无统计学差异（p>0.05）。IOP 与 EVP 之间存在显著的线性相关性（R=0.35，p=0.001）。