病毒载体诱导的小鼠眼内高压。
Viral Vector-Induced Ocular Hypertension in Mice.
机构信息
Department of Pharmacology & Neuroscience, North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA.
Department of Ophthalmology, Gaven Herbert Eye Institute, UC Irvine, Irvine, CA, USA.
出版信息
Methods Mol Biol. 2023;2708:77-97. doi: 10.1007/978-1-0716-3409-7_9.
Viral transduction of the mouse trabecular meshwork using a variety of transgenes associated with glaucoma generates an inducible and reproducible method for generating ocular hypertension due to increased aqueous humor outflow resistance of the conventional outflow pathway. Both adenovirus serotype 5 (Ad5) and lentiviruses have selective tropism for the mouse trabecular meshwork with intraocular injections. Accurate intraocular pressures are easily measured using a rebound tonometer, and aqueous humor outflow facilities can be measured in anesthetized live mice.
使用与青光眼相关的多种转基因病毒对小鼠小梁网进行病毒转导，可产生一种诱导性和可重复性的方法，导致常规流出途径的房水流出阻力增加，从而引起眼内高压。腺病毒血清型 5（Ad5）和慢病毒对眼内注射的小鼠小梁网具有选择性趋向性。使用回弹眼压计可以轻松测量准确的眼压，并且可以在麻醉的活鼠中测量房水流出设施。
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