Department of Cardiology, The Second Hospital of Anhui Medical University, Hefei, China; Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA.
Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Department of Intensive Care Unit, The Second Hospital of Anhui Medical University, Hefei, China.
Biomed Pharmacother. 2021 Jan;133:110970. doi: 10.1016/j.biopha.2020.110970. Epub 2020 Nov 7.
Accumulating evidence demonstrated that administration of ω-3 polyunsaturated fatty acid (ω-3 PUFA) or ascorbic acid (AA) following cardiac arrest (CA) improves survival. Therefore, we investigate the effects of ω-3 PUFA combined with AA on myocardial function after CA and cardiopulmonary resuscitation (CPR) in a rat model. Thirty male rats were randomized into 5 groups: (1) sham; (2) control; (3) ω-3 PUFA; (4) AA; (5) ω-3 PUFA + AA. Ventricular fibrillation (VF) was induced and untreated for 6 min followed by defibrillation after 8 min of CPR. Infusion of drug or vehicle occurred at the start of CPR. Myocardial function and sublingual microcirculation were measured at baseline and after return of spontaneous circulation (ROSC). Heart tissues and blood were collected 6 h after ROSC. Myocardial function and sublingual microcirculation improvements were seen with ω-3 PUFA or AA compared to control after ROSC (p < 0.05). ω-3 PUFA + AA shows a better myocardial function than ω-3 PUFA or AA (p < 0.05). ω-3 PUFA or AA decreases pro-inflammatory cytokines, cTnI, myocardium malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) modified proteins compared to control (p < 0.05). ω-3 PUFA and AA combined have lower MDA and 4-HNE modified proteins than alone (p < 0.05). ω-3 PUFA or AA treatment reduces the severity of post-resuscitation myocardial dysfunction, improves sublingual microcirculation, decreases lipid peroxidation and systemic inflammation in the early phase of recovery following CA and resuscitation. A combination of ω-3 PUFA and AA treatment confers an additive effect in suppressing lipid peroxidation and improving myocardial function.
越来越多的证据表明，心脏骤停 (CA) 后给予ω-3 多不饱和脂肪酸 (ω-3 PUFA) 或抗坏血酸 (AA) 可提高存活率。因此，我们在大鼠模型中研究了 ω-3 PUFA 联合 AA 对 CA 和心肺复苏 (CPR) 后心肌功能的影响。30 只雄性大鼠随机分为 5 组：(1)假手术组；(2)对照组；(3)ω-3 PUFA 组；(4)AA 组；(5)ω-3 PUFA+AA 组。诱导心室颤动 (VF)，不治疗 6 分钟，CPR 8 分钟后除颤。CPR 开始时输注药物或载体。在基线和自主循环恢复 (ROSC) 后测量心肌功能和舌下微循环。ROSC 后 6 小时采集心脏组织和血液。与对照组相比，ROSC 后 ω-3 PUFA 或 AA 组的心肌功能和舌下微循环改善 (p<0.05)。与 ω-3 PUFA 或 AA 相比，ω-3 PUFA+AA 具有更好的心肌功能 (p<0.05)。与对照组相比，ω-3 PUFA 或 AA 降低了促炎细胞因子、cTnI、心肌丙二醛 (MDA) 和 4-羟基壬烯醛 (4-HNE) 修饰蛋白 (p<0.05)。与单独使用相比，ω-3 PUFA 和 AA 联合使用时 MDA 和 4-HNE 修饰蛋白更低 (p<0.05)。ω-3 PUFA 或 AA 治疗可减轻 CA 和复苏后早期再灌注后心肌功能障碍的严重程度，改善舌下微循环，降低脂质过氧化和全身炎症反应。ω-3 PUFA 和 AA 联合治疗在抑制脂质过氧化和改善心肌功能方面具有相加作用。
