一种基于高效半花菁染料的比率型荧光探针，用于活细胞和海水中的二氧化硫衍生物。
An efficient hemicyanine dyes-based ratiometric fluorescence probe for sulfur dioxide derivatives in live-cells and seawater.
机构信息
School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Science, Xiamen University, Xiamen, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119128. doi: 10.1016/j.saa.2020.119128. Epub 2020 Oct 29.
In life systems, sulfurdioxide (SO) has very important function in several physiological processes, which can be generated endogenously during the biosynthesis of sulfur-containing amino acids. Herein, a ratiometric fluorescence probe CY carried with the structure of hemicyanine dyes has been developed to track SO derivatives through Michael-addition reaction. The solution of CY shows significant changes from yellow to colorless after adding the SO/HSO in day light within 2 min. Successfully, probe CY can detect SO derivatives in living cells and seawater. Furthermore, probe CY also be used to monitor the change of SO derivatives in seawater. These results give evidence of the potential application of CY in future biomedical diagnosis and marine environment research.
在生命体系中，二氧化硫（SO）在几个生理过程中具有非常重要的功能，它可以在含硫氨基酸的生物合成过程中内源性产生。在此，我们开发了一种带有半花青染料结构的比率型荧光探针 CY，通过迈克尔加成反应来跟踪 SO 衍生物。在日光下，将 SO/HSO 添加到 CY 的溶液中 2 分钟内，溶液的颜色从黄色显著变为无色。成功地，探针 CY 可以在活细胞和海水中检测 SO 衍生物。此外，探针 CY 还可用于监测海水中 SO 衍生物的变化。这些结果为 CY 在未来生物医学诊断和海洋环境研究中的应用提供了证据。
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