基于半花菁的荧光探针同时检测溶酶体 SO 和粘度。
Simultaneous detection of lysosomal SO and viscosity using a hemicyanine-based fluorescent probe.
机构信息
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China.
College of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Nov 5;280:121519. doi: 10.1016/j.saa.2022.121519. Epub 2022 Jun 17.
The changes in sulfur dioxide and viscosity of lysosomes are significant indicators in physiological processes and the cell microenvironment. This study aimed to synthesize a hemicyanine-based probe for simultaneous detection of SO and viscosity. The probe could not only rationally detect sulfur dioxide in a semi-aqueous solution with high sensitivity (limit of detection = 0.78 μM) and fast response (within 30 s) but also monitor viscosity via fluorescence emission enhancement at 580 nm. Further, the dual-response probe was successfully used to image SO and viscosity in the lysosomes of living cells.
溶酶体中二氧化硫和粘度的变化是生理过程和细胞微环境的重要指标。本研究旨在合成一种基于半花菁的探针，用于同时检测 SO 和粘度。该探针不仅可以在半水相中以高灵敏度（检测限=0.78μM）和快速响应（30 秒内）合理检测二氧化硫，还可以通过在 580nm 处的荧光发射增强来监测粘度。此外，该双响应探针成功地用于活细胞溶酶体中 SO 和粘度的成像。
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