Emerson T E
Department of Experimental Therapeutics, Cutter Biological, Miles Inc., Berkeley, CA 94710.
J Trauma. 1987 Nov;27(11):1261-6. doi: 10.1097/00005373-198711000-00010.
The present study was performed to determine the effects of pretreatment with purified human plasma fibronectin (FN) on survival in rats challenged with Salmonella typhimurium peritonitis or E. coli endotoxemia. The effects on survival of combining FN with immunoglobulin G (IgG) or antithrombin-III (AT-III) were also determined during S. typhimurium peritonitis and E. coli endotoxemia. Permanent survival was increased 15% in the peritonitis group (p less than 0.05) and 15% in the endotoxemic group (p greater than 0.05). There was no enhancement in survival by combining FN with a subprotective dose of IgG preparation. AT-III alone increased survival by 50% over survival in the control group. Combining FN with AT-III increased survival 10% greater than with AT-III alone, which is suggestive of an additive effect. Results from this study suggest that FN provides modest protection during Gram-negative peritonitis or endotoxemia in the rat. Combining FN with AT-III may augment in an additive manner the marked increase in survival observed with AT-III alone in this and previous studies.
本研究旨在确定用纯化的人血浆纤维连接蛋白（FN）预处理对遭受鼠伤寒沙门氏菌腹膜炎或大肠杆菌内毒素血症攻击的大鼠存活率的影响。在鼠伤寒沙门氏菌腹膜炎和大肠杆菌内毒素血症期间，还确定了将FN与免疫球蛋白G（IgG）或抗凝血酶III（AT-III）联合使用对存活率的影响。腹膜炎组的永久存活率提高了15%（p<0.05），内毒素血症组提高了15%（p>0.05）。将FN与亚保护剂量的IgG制剂联合使用并未提高存活率。单独使用AT-III使存活率比对照组提高了50%。将FN与AT-III联合使用使存活率比单独使用AT-III提高了10%，提示有相加作用。本研究结果表明，FN在大鼠革兰氏阴性腹膜炎或内毒素血症期间提供适度保护。在本研究及以往研究中，将FN与AT-III联合使用可能以相加方式增强单独使用AT-III时观察到的存活率显著提高。
