Han Xingfa, Meng Fengyan, Cao Xiaohan, Du Xiaogang, Bu Guixian, Kong Fanli, Huang Anqi, Zeng Xianyin
Isotope Research Lab, Sichuan Agricultural University, Ya'an 625014, People's Republic of China.
Isotope Research Lab, Sichuan Agricultural University, Ya'an 625014, People's Republic of China.
Theriogenology. 2021 Jan 15;160:10-17. doi: 10.1016/j.theriogenology.2020.10.029. Epub 2020 Nov 4.
Follicle-stimulating hormone (FSH) was recently implicated as a novel regulator of fat accumulation. Surgical castration causes high FSH concentrations and increases fat accumulation, whereas immunocastration results in low FSH concentrations and less fat in immunocastrated boars versus barrows. However, detailed information regarding the role of FSH in regulation of fat accumulation in male pigs is unclear. First, expression of FSH receptor was confirmed (real-time quantitative PCR) in subcutaneous and visceral adipose tissues (SAT and VAT, respectively) of boars. Then, surgical castration (high FSH model) was compared to immunocastration (low FSH model) to investigate potential roles of FSH in adipogenesis and fat accumulation. High FSH concentrations after surgical castration activated PPARγ signaling by upregulating expression of CREB (P < 0.05), and then recruited an array of PPARγ target adipogenic genes, including transcription factor (C/EBPα), long-chain fatty acid uptake (LPL), fatty acid de novo synthesis (FASN, ACACA) and lipid droplet formation (PLIN1) in both SAT and VAT, promoting fat accumulation in barrows. In contrast, much lower serum FSH concentrations in immunocastrates attenuated (P < 0.05) expressions of PPARγ and PPARγ target genes in both SAT and VAT, resulting in less fat accumulation in immunocastrated boars versus barrows. We concluded that the substantially elevated FSH concentrations in barrows promoted fat accumulation by activating the PPARγ signaling pathway in adipose tissues, whereas immunocastrates accumulated less fat due to low FSH.
促卵泡激素（FSH）最近被认为是脂肪积累的一种新型调节因子。手术去势会导致FSH浓度升高并增加脂肪积累，而免疫去势则导致FSH浓度降低，与阉割公猪相比，免疫去势公猪的脂肪更少。然而，关于FSH在雄性猪脂肪积累调节中的作用的详细信息尚不清楚。首先，通过实时定量PCR在公猪的皮下和内脏脂肪组织（分别为SAT和VAT）中证实了FSH受体的表达。然后，将手术去势（高FSH模型）与免疫去势（低FSH模型）进行比较，以研究FSH在脂肪生成和脂肪积累中的潜在作用。手术去势后高浓度的FSH通过上调CREB的表达激活PPARγ信号通路（P<0.05），然后在SAT和VAT中募集一系列PPARγ靶标脂肪生成基因，包括转录因子（C/EBPα）、长链脂肪酸摄取（LPL）、脂肪酸从头合成（FASN、ACACA）和脂滴形成（PLIN1），促进阉割公猪的脂肪积累。相比之下，免疫去势猪血清中FSH浓度低得多，减弱了（P<0.05）SAT和VAT中PPARγ及其靶标基因的表达，导致免疫去势公猪比阉割公猪脂肪积累更少。我们得出结论，阉割公猪中大幅升高的FSH浓度通过激活脂肪组织中的PPARγ信号通路促进脂肪积累，而免疫去势猪由于FSH浓度低而脂肪积累较少。
