Krupa Petr, Stepankova Katerina, Kwok Jessica Cf, Fawcett James W, Cimermanova Veronika, Jendelova Pavla, Machova Urdzikova Lucia
Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic.
Department of Neurosurgery, Charles University, Medical Faculty and University Hospital Hradec Králové, Sokolska 581, 50005 Hradec Kralove, Czech Republic.
Biomedicines. 2020 Nov 5;8(11):477. doi: 10.3390/biomedicines8110477.
Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty's catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty´s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL.
尽管目前使用了多种脊髓损伤（SCI）实验模型，但人类常见的腹侧压迫性脊髓损伤模型却非常有限。腹侧球囊压迫损伤反映了人类损伤的常见解剖机制，并且具有通过控制球囊的充气量来对损伤严重程度进行分级的优点。在本研究中，通过椎板切除术，在T10水平经硬膜外前路放置2F Fogarty导管的球囊对脊髓进行腹侧压迫。球囊迅速用10或15μL生理盐水充气，并原位静置5分钟。通过行为学和免疫组织化学测试评估损伤的严重程度。用15μL的体积进行压迫导致严重的运动和感觉缺陷，表现为完全无法在水平梯子上移动、最终的Basso、Beattie和Bresnahan（BBB）评分为7.4以及足底试验中退缩时间缩短（11.6秒）。组织学和免疫组织化学显示白质和灰质显著丢失，运动神经元丧失，星形胶质细胞增生面积增加。10μL的充气量导致轻度短暂缺陷。目前没有其他腹侧脊髓损伤的球囊压迫模型。本研究提供并验证了一种由Fogarty导管的充气球囊引入的新型、易于复制的腹侧压迫性脊髓损伤模型。对于严重的不完全缺陷，充气量应保持在15μL。
