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单细胞转录组在新鲜和冷冻保存人类皮肤组织比较分析中的保守性：局限性硬皮病的初步研究。

Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma.

机构信息

Division of Rheumatology, Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA, USA.

Division of Rheumatology, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

出版信息

Arthritis Res Ther. 2020 Nov 9;22(1):263. doi: 10.1186/s13075-020-02343-4.

DOI:10.1186/s13075-020-02343-4
PMID:33168063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7654179/
Abstract

BACKGROUND

The purpose of this study was to assess variability in cell composition and cell-specific gene expression in the skin of patients with localized scleroderma (LS) utilizing CryoStor® CS10 in comparison to RPMI to produce adequate preservation of tissue samples and cell types of interest for use in large-scale multi-institutional collaborations studying localized scleroderma and other skin disorders.

METHODS

We performed single-cell RNA sequencing on paired skin biopsy specimens from 3 patients with LS. Each patient with one sample cryopreserved in CryoStor® CS10 and one fresh in RPMI media using 10× Genomics sequencing.

RESULTS

Levels of cell viability and yield were comparable between CryoStor® CS10 (frozen) and RPMI (fresh) preserved cells. Furthermore, gene expression between preservation methods was collectively significantly correlated and conserved across all 18 identified cell cluster populations.

CONCLUSION

Comparable cell population and transcript expression yields between CryoStor® CS10 and RPMI preserved cells support the utilization of cryopreserved skin tissue in single-cell analysis. This suggests that employing standardized cryopreservation protocols for the skin tissue will help facilitate multi-site collaborations looking to identify mechanisms of disease in disorders characterized by cutaneous pathology.

摘要

背景

本研究旨在利用 CryoStor® CS10 与 RPMI 比较评估局限性硬皮病（LS）患者皮肤细胞组成和细胞特异性基因表达的变异性，以便为大规模多机构合作研究局限性硬皮病和其他皮肤疾病提供足够的组织样本和感兴趣的细胞类型保存。

方法

我们对 3 例 LS 患者的配对皮肤活检标本进行了单细胞 RNA 测序。每位患者的一份样本保存在 CryoStor® CS10 中冷冻，另一份新鲜的保存在 RPMI 培养基中，使用 10× Genomics 测序。

结果

CryoStor® CS10（冷冻）和 RPMI（新鲜）保存细胞的细胞活力和产率水平相当。此外，两种保存方法之间的基因表达具有显著的相关性和一致性，跨越了所有 18 种鉴定的细胞簇群。

结论

CryoStor® CS10 和 RPMI 保存细胞之间具有可比性的细胞群体和转录表达产量支持在单细胞分析中使用冷冻保存的皮肤组织。这表明采用标准化的皮肤组织冷冻保存方案将有助于促进多站点合作，以鉴定以皮肤病理学为特征的疾病的发病机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/6c268e4c1744/13075_2020_2343_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/c23c1e440324/13075_2020_2343_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/0d82d2fbac08/13075_2020_2343_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/7e26d1502d68/13075_2020_2343_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/6c268e4c1744/13075_2020_2343_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/c23c1e440324/13075_2020_2343_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/0d82d2fbac08/13075_2020_2343_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/7e26d1502d68/13075_2020_2343_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308e/7654179/6c268e4c1744/13075_2020_2343_Fig4_HTML.jpg

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1
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2
New Approaches to Cryopreservation of Cells, Tissues, and Organs.细胞、组织和器官冷冻保存的新方法。
Transfus Med Hemother. 2019 Jun;46(3):197-215. doi: 10.1159/000499453. Epub 2019 Jun 4.
3
Comprehensive Integration of Single-Cell Data.单细胞数据的综合整合。
利用可解释机器学习识别局限性硬皮病发病机制背后独特且共享的转录组特征。
JCI Insight. 2025 Apr 8;10(7):e185758. doi: 10.1172/jci.insight.185758.
4
Single-Cell Transcriptomics Sheds Light on Tumor Evolution: Perspectives from City of Hope's Clinical Trial Teams.单细胞转录组学揭示肿瘤演变：希望之城临床试验团队的观点
J Clin Med. 2024 Dec 10;13(24):7507. doi: 10.3390/jcm13247507.
5
A cell atlas foundation model for scalable search of similar human cells.一种用于可扩展搜索相似人类细胞的细胞图谱基础模型。
Nature. 2025 Feb;638(8052):1085-1094. doi: 10.1038/s41586-024-08411-y. Epub 2024 Nov 20.
6
Characterization of Endothelial Cell Subclusters in Localized Scleroderma Skin with Single-Cell RNA Sequencing Identifies NOTCH Signaling Pathway.单细胞 RNA 测序对局限性硬皮病皮肤中内皮细胞亚群的特征分析显示 NOTCH 信号通路。
Int J Mol Sci. 2024 Sep 28;25(19):10473. doi: 10.3390/ijms251910473.
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