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Hrd1 在紫外线（UV）辐射诱导光老化中的作用。

The role of Hrd1 in ultraviolet (UV) radiation induced photoaging.

机构信息

Department of Dermatology and Venereology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

Department of Pediatric and Preventive Dentistry, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China.

出版信息

Aging (Albany NY). 2020 Nov 9;12(21):21273-21289. doi: 10.18632/aging.103851.

DOI:10.18632/aging.103851
PMID:33168784
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7695362/
Abstract

The purpose of the present study was to evaluate the role of Hrd1 in the ultraviolet (UV) radiation induced photoaging and explore its potential mechanism. The nude mice were exposed to the UVA/UVB irradiation for 10 weeks. The animals were subcutaneously injected with AAV5-NC, Hrd1-shRNA-AAV5, or Hrd1-overexpression-AAV5. The HSF cells were also transfected with Ad-NC, Ad-shRNA-Hrd1, or Ad-Hrd1, and irradiated by UVA/UVB stimulation. The clinical skin samples were harvested for detecting Hrd1 and IGF-1R expressions. As a result, the knockdown of Hrd1 attenuated the histopathological alteration and collagen degradation in UV-induced nude mice. The inhibition of Hrd1 by Hrd1-shRNA-AAV5 and Ad-shRNA-Hrd1 inhibited the Hrd1 expression and promoted IGF-1R, Type I collagen and type III collagen in mice and HSF cells. The overexpression of Hrd1 exerted the reverse effect. The Co-IP assay also indicated the interaction between Hrd1 and IGF-1R. Hrd1-mediated IGF-1R downregulation and collagen degradation were also observed in clinical skin samples. In conclusion, the present results demonstrated that Hrd1 degraded IGF-1R and collagen formation in UV-induced photoaging.

摘要

本研究旨在评估 Hrd1 在紫外线（UV）辐射诱导的光老化中的作用，并探讨其潜在机制。裸鼠接受 UVA/UVB 照射 10 周。动物被皮下注射 AAV5-NC、Hrd1-shRNA-AAV5 或 Hrd1-过表达-AAV5。HSF 细胞也用 Ad-NC、Ad-shRNA-Hrd1 或 Ad-Hrd1 转染，并接受 UVA/UVB 刺激。采集临床皮肤样本检测 Hrd1 和 IGF-1R 的表达。结果表明，Hrd1 的敲低减轻了 UV 诱导的裸鼠的组织病理学改变和胶原降解。Hrd1-shRNA-AAV5 和 Ad-shRNA-Hrd1 抑制 Hrd1 的表达并促进 IGF-1R、I 型胶原和 III 型胶原在小鼠和 HSF 细胞中的表达。Hrd1 的过表达则产生相反的效果。Co-IP 测定也表明了 Hrd1 与 IGF-1R 之间的相互作用。在临床皮肤样本中也观察到 Hrd1 介导的 IGF-1R 下调和胶原降解。总之，本研究结果表明，Hrd1 降解 IGF-1R 和胶原形成在 UV 诱导的光老化中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/a8919f1f02d4/aging-12-103851-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/9e8b41b94f23/aging-12-103851-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/f0419d6e397c/aging-12-103851-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/8f7f88f58cd7/aging-12-103851-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/fbd81a6cda28/aging-12-103851-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/dbad6906abac/aging-12-103851-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/0930dbd2b661/aging-12-103851-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/e1fe16bf7bd8/aging-12-103851-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/3c8e73da8410/aging-12-103851-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/413ee244b439/aging-12-103851-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/a8919f1f02d4/aging-12-103851-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/9e8b41b94f23/aging-12-103851-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/f0419d6e397c/aging-12-103851-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/8f7f88f58cd7/aging-12-103851-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/fbd81a6cda28/aging-12-103851-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/dbad6906abac/aging-12-103851-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/0930dbd2b661/aging-12-103851-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/e1fe16bf7bd8/aging-12-103851-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/3c8e73da8410/aging-12-103851-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/413ee244b439/aging-12-103851-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa9e/7695362/a8919f1f02d4/aging-12-103851-g010.jpg

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本文引用的文献

1
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J Photochem Photobiol B. 2020 Jan;202:111720. doi: 10.1016/j.jphotobiol.2019.111720. Epub 2019 Nov 23.
2
A technique for delineating the unfolding requirements for substrate entry into retrotranslocons during endoplasmic reticulum-associated degradation.一种描绘内质网相关降解过程中底物进入逆行转位复合体所需展开的要求的技术。
J Biol Chem. 2019 Dec 27;294(52):20084-20096. doi: 10.1074/jbc.RA119.010019. Epub 2019 Nov 20.
3
Antioxidant cinnamaldehyde attenuates UVB-induced photoaging.抗氧化剂肉桂醛可减轻 UVB 诱导的光老化。
J Dermatol Sci. 2019 Dec;96(3):151-158. doi: 10.1016/j.jdermsci.2019.11.001. Epub 2019 Nov 6.
4
Protective effects of galangin against H O -induced aging via the IGF-1 signaling pathway in human dermal fibroblasts.高良姜素通过IGF-1信号通路对过氧化氢诱导的人皮肤成纤维细胞衰老的保护作用。
Environ Toxicol. 2020 Feb;35(2):115-123. doi: 10.1002/tox.22847. Epub 2019 Sep 30.
5
Asiatic Acid Glucosamine Salt Alleviates Ultraviolet B-induced Photoaging of Human Dermal Fibroblasts and Nude Mouse Skin.氨基葡萄糖盐亚洲酸减轻紫外线 B 诱导的人真皮成纤维细胞和裸鼠皮肤光老化。
Photochem Photobiol. 2020 Jan;96(1):124-138. doi: 10.1111/php.13160. Epub 2019 Oct 17.
6
ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection.内质网定位的 Hrd1 泛素化并失活 Usp15，以促进细菌感染时 TLR4 诱导的炎症反应。
Nat Microbiol. 2019 Dec;4(12):2331-2346. doi: 10.1038/s41564-019-0542-2.
7
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Proc Natl Acad Sci U S A. 2019 Mar 5;116(10):4722-4731. doi: 10.1073/pnas.1816268116. Epub 2019 Feb 20.