胃肠道间质瘤糖脂的特征分析。

Characterization of glycosphingolipids from gastrointestinal stromal tumours.

机构信息

Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, P.O. Box 440, 405 30, Gothenburg, Sweden.

Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

出版信息

Sci Rep. 2020 Nov 9;10(1):19371. doi: 10.1038/s41598-020-76104-3.

DOI:10.1038/s41598-020-76104-3

PMID:33168837

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7653041/

Abstract

Gastrointestinal stromal tumours (GISTs) are the major nonepithelial neoplasms of the human gastrointestinal tract with a worldwide incidence between 11 and 15 per million cases annually. In this study the acid and non-acid glycosphingolipids of three GISTs were characterized using a combination of thin-layer chromatography, chemical staining, binding of carbohydrate recognizing ligands, and mass spectrometry. In the non-acid glycosphingolipid fractions of the tumors globotetraosylceramide, neolactotetraosylceramide, and glycosphingolipids with terminal blood group A, B, H, Le, Le, Le and Le determinants were found. The relative amounts of these non-acid compounds were different in the three tumour samples. The acid glycosphingolipid fractions had sulfatide, and the gangliosides GM3, GD3, GM1, Neu5Acα3neolactotetraosylceramide, GD1a, GT1b and GQ1b. In summary, we have characterized the glycosphingolipids of GISTs and found that the pattern differs in tumours from different individuals. This detailed characterization of glycosphingolipid composition of GISTs could contribute to recognition of new molecular targets for GIST treatment and sub-classification.

摘要

胃肠道间质肿瘤（GISTs）是人类胃肠道的主要非上皮性肿瘤，每年全球发病率为每百万例 11-15 例。在这项研究中，我们使用薄层层析、化学染色、糖识别配体结合以及质谱联用的方法，对三种 GIST 的酸性和非酸性糖脂进行了特征描述。在肿瘤的非酸性糖脂馏分中，发现了Globotetraosylceramide、Neolactotetraosylceramide 和具有末端血型 A、B、H、Le、Le、Le 和 Le 决定簇的糖脂。这三种肿瘤样本中的这些非酸性化合物的相对含量不同。酸性糖脂馏分含有硫酸酯和神经节苷脂 GM3、GD3、GM1、Neu5Acα3Neolactotetraosylceramide、GD1a、GT1b 和 GQ1b。总之，我们已经对 GIST 的糖脂进行了特征描述，并发现不同个体的肿瘤中的模式存在差异。对 GIST 糖脂组成的这种详细特征描述可能有助于识别 GIST 治疗和亚类的新分子靶标。