a Department of Medical Biochemistry and Cell Biology , Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg , Gothenburg , Sweden.
Virulence. 2018 Dec 31;9(1):738-751. doi: 10.1080/21505594.2018.1440171.
Adhesion of Helicobacter pylori to the gastric mucosa is a prerequisite for the pathogenesis of H. pylori related diseases. In this study, we investigated the ganglioside composition of human stomach as the target for attachment mediated by H. pylori SabA (sialic acid binding adhesin). Acid glycosphingolipids were isolated from human stomach and separated into subfractions, which were characterized by mass spectrometry and by binding of antibodies, bacteria, and Solanum tuberosum lectin. H. pylori SabA binding gangliosides were characterized as Neu5Acα3-neolactohexaosylceramide and Neu5Acα3-neolactooctaosylceramide, while the other acid human stomach glycosphingolipids characterized (sulfatide and the gangliosides GM3, GD3, GM1, Neu5Acα3-neolactotetraosylceramide, GD1a and GD1b) were not recognized by the bacteria. Defining H. pylori binding glycosphingolipids of the human gastric mucosa will be useful to specifically target this microbe-host interaction for therapeutic intervention.
幽门螺杆菌（Helicobacter pylori）黏附于胃黏膜是其引发相关疾病的前提条件。在本研究中，我们研究了人胃的神经节苷脂组成，作为幽门螺杆菌 SabA（唾液酸结合黏附素）介导黏附的靶标。从人胃中分离酸性糖脂，并将其分为亚组分，通过质谱法和抗体、细菌以及茄属 Solanum tuberosum 凝集素的结合对其进行鉴定。幽门螺杆菌 SabA 结合的神经节苷脂被鉴定为 Neu5Acα3-新乳糖六糖神经酰胺和 Neu5Acα3-新乳糖八糖神经酰胺，而其他鉴定的人胃酸性糖脂（硫酸脑苷脂和神经节苷脂 GM3、GD3、GM1、Neu5Acα3-新乳糖四糖神经酰胺、GD1a 和 GD1b）则未被细菌识别。确定人胃黏膜中幽门螺杆菌的结合糖脂将有助于针对这种微生物-宿主相互作用进行特异性的治疗干预。
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