Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, China.
Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Biomed Chromatogr. 2021 Apr;35(4):e5024. doi: 10.1002/bmc.5024. Epub 2020 Nov 26.
This study was designed to explore the bioactive ingredients in the extracts of Fallopia denticulata (C.C. Huang) Holub, a medicinal plant grown in China, which exhibits the best neuraminidase (NA) inhibition activity. Three fractions of ethyl acetate, ethanol, and water were tested on NA inhibition assay, and the best one was conducted by ultra-performance liquid chromatography-time-of-flight mass spectrometry in the negative and positive modes to analyze the metabolic components. The results revealed the identification of the following 21 compounds: 3 organic acids, 11 flavonoids, 1 coumarin, and 6 others, such as β-daucosterol, gallic acid, and syringic acid, of which 12 compounds were discovered for the first time in F. denticulata. In addition, we used the molecular docking technique to support the anti-NA activity of each compound in the best extract. The results confirmed that the two better bioactive compounds were (-)-epicatechin gallate and (+)-catechin. Therefore, F. denticulata could be used as a potential material for new anti-influenza drugs.
本研究旨在探索中国产药用植物虎杖（Fallopia denticulata (C.C. Huang）的提取物中的生物活性成分，该植物具有最佳的神经氨酸酶（NA）抑制活性。我们对虎杖的乙酸乙酯、乙醇和水三种提取物进行了 NA 抑制活性测定，结果显示其中乙酸乙酯提取物的抑制活性最佳，然后我们采用超高效液相色谱-飞行时间质谱法（UPLC-TOF-MS）在正负模式下对其代谢成分进行分析。结果共鉴定出 21 种化合物：3 种有机酸、11 种黄酮类化合物、1 种香豆素和 6 种其他化合物，如β-豆甾醇、没食子酸和丁香酸，其中 12 种化合物为首次在虎杖中发现。此外，我们还使用分子对接技术来支持最佳提取物中每种化合物的抗 NA 活性。结果证实，两种更好的生物活性化合物为（-）-表儿茶素没食子酸酯和（+）-儿茶素。因此，虎杖可作为新型抗流感药物的潜在材料。
