Department of Medical Pathological Techniques, Marmara University, Vocational School of Health Services, İstanbul, Turkey.
Department of Physiology, Atlas University School of Medicine, İstanbul, Turkey.
Turk J Gastroenterol. 2020 Oct;31(10):706-712. doi: 10.5152/tjg.2020.19520.
BACKGROUND/AIMS: Astaxanthin (ATX) is a naturally occurring carotenoid and a potent antioxidant. Various anti-inflammatory effects of ATX have been examined. We aimed to investigate the protective effect of ATX and its mechanism in a cerulein-induced acute pancreatitis rat model.
The rats were randomized into 2 main groups as control (C) and acute pancreatitis group (AP). AP group was subsequently divided into subgroups as AP+vehicle (AP), AP+ATX, and ATX+peroxisome proliferator-activated receptor-alpha antagonist GW6471 (ATX+GW) groups. To induce AP, the rats were administered cerulein (50 µg/kg, intraperitonally [ip]) at 1 hour intervals, whereas the C group received saline. The AP group was treated with vehicle olive oil, ATX 40 mg/kg/orally, or GW6471 and ATX (GW1 mg/kg/ip; ATX; 40 mg/kg/peroral). Treatments were administered after the 1st cerulein injection. At the 7th hour after the final injection, the rats were killed and the pancreatic tissue was used for the determination of malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO) activities and luminol-lucigenin chemiluminescence levels. Serum amylase, lipase, and histopathological analyses were performed.
Elevated serum lipase and amylase levels in the vehicle-treated AP group (p<0.01) decreased in the ATX and ATX+GW groups (p<0.05). In the AP groups, GSH was reduced and MDA, MPO, luminol, and lucigenin levels were increased (p<0.05-0.001). ATX reversed these changes (p<0.05-0.001). The vehicle-treated group revealed significant severe cytoplasmic degeneration and vacuolization, whereas ATX ameliorated these destructions. GW6471 did not abolish the positive effects of ATX biochemically or histologically.
ATX has a potent protective effect on AP via its radical scavenging and antioxidant properties. Therefore, we believe that ATX may have therapeutic potential.
背景/目的：虾青素（ATX）是一种天然存在的类胡萝卜素，也是一种有效的抗氧化剂。已经研究了 ATX 的各种抗炎作用。我们旨在研究 ATX 在雨蛙肽诱导的急性胰腺炎大鼠模型中的保护作用及其机制。
大鼠随机分为对照组（C）和急性胰腺炎组（AP）两个主要组。AP 组随后分为以下亚组：AP+载体（AP）、AP+ATX 和 ATX+过氧化物酶体增殖物激活受体-α拮抗剂 GW6471（ATX+GW）组。为了诱导 AP，大鼠每 1 小时腹膜内（ip）给予雨蛙肽（50 µg/kg），而 C 组给予生理盐水。AP 组给予载体橄榄油、ATX 40 mg/kg/口服、或 GW6471 和 ATX（GW1 mg/kg/ip；ATX；40 mg/kg/口服）。在第一次雨蛙肽注射后给予治疗。最后一次注射后 7 小时，处死大鼠，用胰腺组织测定丙二醛（MDA）、谷胱甘肽（GSH）和髓过氧化物酶（MPO）活性和鲁米诺-荧光素化学发光水平。进行血清淀粉酶、脂肪酶和组织病理学分析。
载体处理的 AP 组的血清脂肪酶和淀粉酶水平升高（p<0.01），而 ATX 和 ATX+GW 组则降低（p<0.05）。在 AP 组中，GSH 减少，MDA、MPO、鲁米诺和荧光素水平增加（p<0.05-0.001）。ATX 逆转了这些变化（p<0.05-0.001）。载体处理的组显示出明显的严重细胞质变性和空泡化，而 ATX 则改善了这些破坏。GW6471 并未在生化或组织学上消除 ATX 的积极作用。
ATX 通过其自由基清除和抗氧化特性对 AP 具有强大的保护作用。因此，我们相信 ATX 可能具有治疗潜力。
