Bao Y, He W, Zhao S, Liu Q, Li K, He P, Li K
College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, China.
Lett Appl Microbiol. 2021 Mar;72(3):341-350. doi: 10.1111/lam.13426. Epub 2020 Dec 1.
An integrated multi-trophic aquaculture (IMTA) system, with one fish cage model surrounded by an island and shellfish rafts, was used in the current study. Planktonic and sediment bacterial communities in the IMTA system were monitored over four seasons in 2019. In both plankton and sediment samples, the most dominant phyla were Proteobacteria and Bacteroidota. Sediment bacterial samples were more similar and had higher levels of biodiversity than planktonic bacterial samples. Obvious seasonal variations were found in plankton samples, but not in sediment samples. No obvious inter-site variations in planktonic and sediment bacteria (fish cages, shellfish rafts and control sites) were found and the results suggested that no obvious impact of feeding operations in fish culture cage model on bacterial communities in the IMTA system was observed in this study. Based on the sequence data, some faecal indicator bacteria and potentially pathogenic bacterial species were detected. According to the results, the bacterial water quality in the IMTA system was acceptable. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) analysis revealed that the primary difference in potential functional roles of planktonic and sediment bacteria was amino acid transport and metabolism, which was active in different seasons.
本研究采用了一种综合多营养层次水产养殖（IMTA）系统，该系统有一个被岛屿和贝类筏包围的鱼笼模型。2019年对IMTA系统中的浮游和沉积物细菌群落进行了四季监测。在浮游和沉积物样本中，最主要的门类是变形菌门和拟杆菌门。沉积物细菌样本比浮游细菌样本更相似，且生物多样性水平更高。浮游样本中发现了明显的季节变化，但沉积物样本中未发现。在浮游和沉积物细菌（鱼笼、贝类筏和对照位点）中未发现明显的位点间差异，结果表明本研究未观察到鱼养殖笼模型中的投喂操作对IMTA系统中的细菌群落有明显影响。基于序列数据，检测到了一些粪便指示菌和潜在致病细菌物种。根据结果，IMTA系统中的细菌水质是可接受的。PICRUSt（通过未观察状态重建对群落进行系统发育研究）分析表明，浮游和沉积物细菌潜在功能作用的主要差异在于氨基酸转运和代谢，其在不同季节活跃。
