Villarroya S, Mérand B, Scholler R
Fondation de Recherche en Hormonologie, Fresnes, France.
Pathol Biol (Paris). 1987 Oct;35(8):1155-9.
A sialoglycoprotein, an integral component of the head plasma membrane of human spermatozoa, is recognized by the a-HS 1A.1 monoclonal antibody. The antigenicity is associated with the sugar moiety since: a) trypsin digestion did not affect the antigenic determinant; b) pretreatment of the cells with beta-glucosidase, alpha-mannosidase and neuraminidase completely abolished antibody binding. Endoglycosidase D and glycopeptidase F were inactive. The a-HS 1A.1 did not recognize a variety of blood-group related synthetic oligosaccharides. The species specificity was studied by indirect immunofluorescence assay. The antibody also recognized an antigen on Macaca fascicularis sperm, but failed to bind to spermatozoa of boar, bull, goat, ram, stallion, dog, rabbit, rooster, carp and eel.
一种唾液糖蛋白是人类精子头部质膜的一个组成部分，可被a-HS 1A.1单克隆抗体识别。这种抗原性与糖部分相关，原因如下：a) 胰蛋白酶消化不影响抗原决定簇；b) 用β-葡萄糖苷酶、α-甘露糖苷酶和神经氨酸酶对细胞进行预处理可完全消除抗体结合。内切糖苷酶D和糖肽酶F无活性。a-HS 1A.1不识别多种与血型相关的合成寡糖。通过间接免疫荧光测定法研究了物种特异性。该抗体也识别食蟹猴精子上的一种抗原，但不能与猪、公牛、山羊、公羊、种马、狗、兔子、公鸡、鲤鱼和鳗鱼的精子结合。
