Czuppon A B
Biochem Int. 1984 Jan;8(1):9-18.
Four O-glycosidic oligosaccharides (I, II, III, IV) were purified from a human spermatozoal sialoglycoprotein antigen following quantitative beta-elimination and reduction of the linkage sugar by KHB4+. The four oligosaccharides showed a similar carbohydrate composition consisting of sialic acids, galactose, fucose, galactosamine and N-acetylgalactosamine, except for the lack of fucose in II. The linkage sugar was identified as N-acetylgalactosamine at a molar ratio of 23.8 to 1 glycoprotein. The sialic acid moieties of the oligosaccharides were tentatively identified by gas-liquid chromatography following trimethylsilylation. A biological function for these sialic acids is suggested by a significantly increased antibody binding after desialization, as compared to the intact sialoglycoprotein molecule. This masking effect of the sialic acids could raise important questions about the etiology of the naturally occurring antispermatozoal antibodies in sera of sterile man, about which is little known.
从人精子涎酸糖蛋白抗原中，通过KHB4 +对连接糖进行定量β-消除和还原，纯化出四种O-糖苷寡糖（I、II、III、IV）。除了II中缺乏岩藻糖外，这四种寡糖显示出相似的碳水化合物组成，包括唾液酸、半乳糖、岩藻糖、半乳糖胺和N-乙酰半乳糖胺。连接糖被鉴定为N-乙酰半乳糖胺，与糖蛋白的摩尔比为23.8比1。寡糖的唾液酸部分在三甲基硅烷化后通过气液色谱法初步鉴定。与完整的涎酸糖蛋白分子相比，去唾液酸化后抗体结合显著增加，这表明这些唾液酸具有生物学功能。唾液酸的这种掩盖作用可能会引发关于不育男性血清中天然存在的抗精子抗体病因的重要问题，而对此人们知之甚少。
