Department of Human Physiology, Vidyasagar University, Midnapore, West Bengal, India.
Department of Anaesthesia and Critical Care, University of Würzburg, Würzburg, Germany.
Biofouling. 2020 Sep;36(8):1000-1017. doi: 10.1080/08927014.2020.1842877. Epub 2020 Nov 10.
Benzyl isocyanate (BIC), from methanol extract of leaves, exhibited substantial anti-biofilm activities against , the common bacterial pathogen in nosocomial infections. Major components of the extract included eugenol, BIC, phenyl-2-methoxy-4-(1-propenyl)-acetate and 2,5-pyrrolidinedione,1-penta-3-4-dienyl, analyzed by GC-MS and HPLC studies. BIC exhibited substantial anti-biofilm activitiy against , established by assaying biofilm formation, biofilm metabolic activity, bacterial adherence to hydrocarbons, exopolysaccharide formation, and optical and scanning electron microscopic studies. BIC significantly downregulated the important biofilm markers of , AD, A and , observed by quantitative real time polymerase chain reaction analysis. Molecular docking studies revealed thermodynamically favorable interaction of BIC with IcaA, SarA and Agr, having Gibbs energy values of -8.45, -9.09 and -10.29 kcal mol, respectively. BIC after binding to IcaR, the repressor of IcaA, influences its binding to target DNA site (, -157.27 kcal mol). The results are considered to demonstrate anti-biofilm potential of BIC against bacterial infections.
苄基异氰酸酯（BIC），来自 的甲醇提取物，对医院感染中常见的细菌病原体 表现出显著的抗生物膜活性。提取物的主要成分包括丁香酚、BIC、苯-2-甲氧基-4-（1-丙烯基）-乙酸酯和 2,5-吡咯烷二酮、1-戊-3-4-二烯基，通过 GC-MS 和 HPLC 研究分析。BIC 通过测定生物膜形成、生物膜代谢活性、细菌对碳氢化合物的粘附、胞外多糖形成以及光学和扫描电子显微镜研究，对 建立的生物膜表现出显著的抗生物膜活性。BIC 通过定量实时聚合酶链反应分析观察到对 的重要生物膜标志物 AD、A 和 的显著下调。分子对接研究表明，BIC 与 IcaA、SarA 和 Agr 具有热力学有利的相互作用，其吉布斯自由能值分别为-8.45、-9.09 和-10.29 kcal mol。BIC 与 IcaA 的抑制剂 IcaR 结合后，影响其与靶 DNA 位点（-157.27 kcal mol）的结合。结果表明 BIC 具有抗细菌感染的抗生物膜潜力。
