Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Department of Biology, Faculty of Sciences, University of Laghouat, Laghouat 03000, Algeria.
ScientificWorldJournal. 2024 Oct 3;2024:6238852. doi: 10.1155/2024/6238852. eCollection 2024.
Medicinal plants are rich sources of bioactive compounds with diverse pharmacological properties, including antimicrobial activities. This study aimed to assess the antibiofilm potential of methanol and ethanol extracts from nine selected medicinal plants, as well as their synergistic effects with doxycycline against strains. Standard procedures were employed to determine the phytochemical composition, total phenolic, and flavonoid contents of the extracts. The antibacterial activity was evaluated using the broth microdilution method, while biofilm formation was assessed via the microtiter plate method. Antibiofilm activity was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide (MTT) assay. Combination studies were conducted using the checkerboard microdilution method. All extracts contained phenols, flavonoids, steroids, triterpenes, and anthraquinones. The methanolic extract of exhibited the highest total phenolic and flavonoid contents (90.48 ± 0.55 mg GAE/g), while the ethanolic extract of showed the highest flavonoid content (6.48 ± 0.33 mg QE/g). Ethanol extracts of and and methanolic extract of demonstrated significant antibacterial activity against 34F2 Sterne strains, with a MIC value of 64 g/mL. Biofilm formation in strains was notably enhanced in the presence of glucose. The methanolic extract of exhibited the highest biofilm imbibition (85%), while methanolic extract showed the most effective biofilm eradication (79%). The combination of ethanol extract with doxycycline displayed synergistic effects against biofilm formation inhibition and eradication in all tested strains. Taken together, ethanol extract shows promise for developing new combination antibacterial therapies.
药用植物是具有多种药理活性的生物活性化合物的丰富来源，包括抗菌活性。本研究旨在评估 9 种选定药用植物甲醇和乙醇提取物的抗生物膜潜力，以及它们与强力霉素对 菌株的协同作用。采用标准程序测定提取物的植物化学成分、总酚和类黄酮含量。采用肉汤微量稀释法测定抗菌活性，采用微量滴定板法评估生物膜形成。采用 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴盐（MTT）测定法测定抗生物膜活性。采用棋盘微量稀释法进行组合研究。所有提取物均含有酚类、类黄酮、类固醇、三萜类和蒽醌类化合物。 甲醇提取物总酚和类黄酮含量最高（90.48±0.55mg GAE/g），而 乙醇提取物类黄酮含量最高（6.48±0.33mg QE/g）。 和 的乙醇提取物和 的甲醇提取物对 34F2 斯特恩菌株表现出显著的抗菌活性，MIC 值为 64μg/mL。在葡萄糖存在下， 菌株的生物膜形成明显增强。 甲醇提取物的生物膜吸光度最高（85%），而 甲醇提取物的生物膜清除率最高（79%）。 乙醇提取物与强力霉素联合使用对所有测试 菌株的生物膜形成抑制和清除均显示协同作用。综上所述， 乙醇提取物具有开发新的联合抗菌治疗方法的潜力。
