Zeng Haiyan, Zhang Xiaolei, Wang Wenqiang, Shen Zhiwei, Dai Zhuozhi, Yu Zhijia, Xu Shuqin, Yan Gen, Huang Qingjun, Wu Renhua, Chen Xi, Xu Haiyun
The Mental Health Center, Shantou University Medical College, Shantou, China.
Xianyue Hospital/Xiamen Mental Health Center, Xiamen, China.
Sci Rep. 2020 Nov 10;10(1):19440. doi: 10.1038/s41598-020-76640-y.
Astrocytes and oligodendrocytes play essential roles in regulating neural signal transduction along neural circuits in CNS. The perfect coordination of neuron/astrocyte and neuron/oligodendrocyte entities was termed as neuron-glia integrity recently. Here we monitored the status of neuron-glia integrity via non-invasive neuroimaging methods and demonstrated the substructures of it using other approaches in an animal model of maternal separation with early weaning (MSEW), which mimics early life neglect and abuse in humans. Compared to controls, MSEW rats showed higher glutamate level, but lower GABA in prefrontal cortex (PFC) detected by chemical exchange saturation transfer and H-MRS methods, lower levels of glial glutamate transporter-1 and ATP-α, but increased levels of glutamate decarboxylase-65 and glutamine synthetase in PFC; reduced fractional anisotropy in various brain regions revealed by diffusion tensor imaging, along with increased levels of N-acetyl-aspartate measured by H-MRS; and hypomyelination in PFC as evidenced by relevant cellular and molecular changes.
星形胶质细胞和少突胶质细胞在调节中枢神经系统（CNS）神经回路中的神经信号转导方面发挥着重要作用。最近，神经元/星形胶质细胞和神经元/少突胶质细胞实体之间的完美协调被称为神经元-胶质细胞完整性。在这里，我们通过非侵入性神经成像方法监测了神经元-胶质细胞完整性的状态，并在早期断奶母鼠分离（MSEW）的动物模型中使用其他方法展示了其亚结构，该模型模拟了人类早期生活中的忽视和虐待。与对照组相比，通过化学交换饱和转移和氢磁共振波谱（H-MRS）方法检测发现，MSEW大鼠前额叶皮质（PFC）中的谷氨酸水平较高，但γ-氨基丁酸（GABA）水平较低；PFC中胶质谷氨酸转运体-1和ATP-α水平较低，但谷氨酸脱羧酶-65和谷氨酰胺合成酶水平升高；扩散张量成像显示不同脑区的各向异性分数降低，同时H-MRS测量的N-乙酰天门冬氨酸水平升高；相关细胞和分子变化证明PFC存在髓鞘形成不足。
