Department of Anesthesiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China.
Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
Mol Med Rep. 2020 Dec;22(6):4821-4827. doi: 10.3892/mmr.2020.11546. Epub 2020 Sep 28.
Chronic postsurgical pain (CPSP) has a high incidence, but the underlying mechanism is not well understood. Accumulating evidence has suggested that central sensitization is the main mechanism of pain. To study the role of p120 in CPSP, a skin/muscle incision and retraction (SMIR) model was established, and immunofluorescence staining and western blotting were performed to analyze the expression of p120 in the spinal cord and dorsal root ganglion (DRG). The results demonstrated that SMIR increased the expression of p120 in the DRG and the spinal cord compared with the naive group. Furthermore, it was demonstrated that p120 was mainly distributed in the glial fibrillary acidic protein‑positive astrocytes in the spinal cord, and in the neurofilament 200‑positive medium and large neurons in the DRG. Our previous studies have shown that adenosine triphosphate‑sensitive potassium channel (KATP) agonists can reduce postoperative pain in rats. Therefore, the changes in p120 were observed in the DRG and spinal cord of rats following the intraperitoneal injection of nicorandil, a KATP agonist. It was demonstrated that nicorandil administration could relieve mechanical pain experienced following SMIR in rats, and decrease the expression of p120 in the DRG and spinal cord. The results revealed that p120 may contribute to the prophylactic analgesic effect of nicorandil, thus providing a novel insight into the mechanism of CPSP prevention.
慢性术后疼痛（CPSP）发病率较高，但发病机制尚不清楚。越来越多的证据表明，中枢敏化是疼痛的主要机制。为了研究 p120 在 CPSP 中的作用，建立了皮肤/肌肉切开和牵拉（SMIR）模型，并通过免疫荧光染色和 Western blot 分析脊髓和背根神经节（DRG）中 p120 的表达。结果表明，与正常组相比，SMIR 增加了 DRG 和脊髓中 p120 的表达。此外，结果表明 p120 主要分布在脊髓中胶质纤维酸性蛋白阳性星形胶质细胞中，以及 DRG 中神经丝 200 阳性的中大型神经元中。我们之前的研究表明，三磷酸腺苷敏感性钾通道（KATP）激动剂可减轻大鼠术后疼痛。因此，观察了腹腔注射 KATP 激动剂尼克诺地平后 DRG 和脊髓中 p120 的变化。结果表明，尼克诺地平给药可缓解 SMIR 后大鼠的机械痛，并降低 DRG 和脊髓中 p120 的表达。结果表明，p120 可能有助于尼克诺地平的预防性镇痛作用，从而为 CPSP 预防的机制提供了新的见解。
