Department of Plastic and Reconstructive Surgery, Ajou University School of Medicine, 164, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, 16499, Republic of Korea.
Aesthetic Plast Surg. 2021 Feb;45(1):322-331. doi: 10.1007/s00266-020-02033-1. Epub 2020 Nov 10.
Biphasic hyaluronic acid (HA) fillers have been used extensively to improve facial skin. However, in some cases, the skin surface is irregular because of the premature loss of HA solution. We propose a new biphasic filler (G-filler) to overcome this problem by using small particles of HA hydrogel instead of HA solution, which can provide a smooth skin surface and good durability.
We evaluated the rheologic properties of G-filler and its physiologic effects after subcutaneous injection in a mouse model by histologic analysis.
The G-filler showed a similar elastic modulus (G') and complex viscosity (η*) as the conventional biphasic filler, but had a higher viscous modulus (G″) than the conventional monophasic filler. The highest material elasticity (tan δ) value and the lowest percentage elasticity value indicate the rheologic properties of G-filler are closer to those of liquids. After subcutaneous injection of G-filler, collagen content (~ 2-fold) and elastin fibers (~ 6.5-fold) were significantly increased at 12 weeks compared to those of the saline group. Fibronectin (~ 2.6-fold) and the laminin-immunolabeled cell number (~ 6-fold) were also significantly increased at 12 weeks. Significant increases in the CD31-immunoreactive cell numbers of the G-filler groups were observed at 2, 6, and 12 weeks (~ 3.7-fold) compared to those of the saline groups. There were no significant differences between the G-filler and saline groups in patterns of skin thickness and inflammatory cell numbers around loading sites.
These findings demonstrate that the injection of a new biphasic filler with improved rheologic properties can effectively stimulate extracellular matrix production and angiogenesis without safety concerns.
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双相透明质酸（HA）填充剂已广泛用于改善面部皮肤。然而，在某些情况下，由于 HA 溶液过早流失，皮肤表面会变得不规则。我们提出了一种新的双相填充剂（G-填充剂），通过使用小的 HA 水凝胶颗粒代替 HA 溶液来克服这个问题，这可以提供光滑的皮肤表面和良好的耐用性。
我们通过组织学分析评估了 G-填充剂的流变学特性及其在小鼠模型中的生理效应。
G-填充剂表现出与传统双相填充剂相似的弹性模量（G'）和复粘度（η*），但粘性模量（G″）高于传统单相填充剂。最高的材料弹性（tan δ）值和最低的弹性百分比值表明 G-填充剂的流变学性质更接近液体。与生理盐水组相比，G-填充剂皮下注射后 12 周时胶原含量（2 倍）和弹性纤维（6.5 倍）显著增加。纤维连接蛋白（2.6 倍）和层粘连蛋白免疫标记细胞数（6 倍）也在 12 周时显著增加。G-填充剂组的 CD31 免疫反应性细胞数在 2、6 和 12 周时（~3.7 倍）明显高于生理盐水组。G-填充剂组和生理盐水组在载物部位周围的皮肤厚度和炎症细胞数量的模式上没有显著差异。
这些发现表明，注射具有改善流变学特性的新型双相填充剂可以有效地刺激细胞外基质的产生和血管生成，而不会产生安全性问题。
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