Kokai Y, Cohen J A, Drebin J A, Greene M I
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8498-501. doi: 10.1073/pnas.84.23.8498.
The neu oncogene (also referred to as c-erbB-2 and HER2) encodes a 185-kDa transmembrane glycoprotein with tyrosine kinase activity termed p185. The p185 glycoprotein is structurally related to the epidermal growth factor receptor. It is thought that p185 is the receptor for an as yet unidentified growth factor. In the present study, RNA blot analyses and immunohistochemical studies were performed on rat tissues obtained from a variety of prenatal and postnatal stages to examine the expression of the neu oncogene and its product, p185, during normal development. Expression of the neu gene was detected in mid-gestation embryos in a variety of tissues including nervous system, connective tissue, and secretory epithelium, but not in lymphoid tissue. In adult animals, secretory epithelial tissues and basal cells of the skin expressed neu. These studies demonstrate that the neu gene is expressed in a tissue- and developmental stage-specific manner. We suggest that the p185 molecule plays an important role in the growth and development of a variety of tissues, and, in particular, in epithelial tissue.
神经癌基因（也称为c-erbB-2和HER2）编码一种具有酪氨酸激酶活性的185 kDa跨膜糖蛋白，称为p185。p185糖蛋白在结构上与表皮生长因子受体相关。据认为，p185是一种尚未确定的生长因子的受体。在本研究中，对从各种产前和产后阶段获得的大鼠组织进行了RNA印迹分析和免疫组织化学研究，以检查神经癌基因及其产物p185在正常发育过程中的表达。在妊娠中期胚胎的各种组织中检测到神经癌基因的表达，包括神经系统、结缔组织和分泌上皮，但在淋巴组织中未检测到。在成年动物中，分泌上皮组织和皮肤基底细胞表达神经癌基因。这些研究表明，神经癌基因以组织和发育阶段特异性的方式表达。我们认为，p185分子在多种组织的生长和发育中起重要作用，尤其是在上皮组织中。
