College of Food and Bioengineering, Henan University of Science and Technology, 471023, Luoyang, Henan, P. R. China.
Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 200127, Shanghai, P. R. China.
Chembiochem. 2021 Apr 6;22(7):1210-1214. doi: 10.1002/cbic.202000640. Epub 2020 Dec 9.
DNA with genetic information was edited to regulate and repair the structure and function of a protein. In DNA nanotechnology, DNA with programmable information can be designed to edit the fluorescence intensity and emissive colors of DNA-stabilized silver nanoclusters (DNA/AgNCs). By introducing and moving one cytosine in the spacer of the emitter domain, we have built up a simple strategy to regulate the excitation and emission wavelengths of AgNCs. When replacing thymine in the spacer of the emitter with one cytosine, the expected excitation and emission change do not occur. However, after moving the introduced cytosine, DNA templates produce AgNCs with extremely different excitation and emission wavelengths from those of the initial template, leading to a template for near-infrared (NIR) emissive species with the highest fluorescence intensity. The formation of AgNCs induces the DNA template into condensed secondary structure based on an altered migration rate in PAGE. The simple strategy of moving one cytosine in a spacer in the emitter domain can enrich the library of templates for synthesizing diverse DNA/AgNCs and has great potential in bioimaging and probe design.
对携带有遗传信息的 DNA 进行编辑，以调节和修复蛋白质的结构和功能。在 DNA 纳米技术中，可以设计具有可编程信息的 DNA 来编辑 DNA 稳定的银纳米团簇（DNA/AgNCs）的荧光强度和发射颜色。通过在发射体域的间隔区引入并移动一个胞嘧啶，我们构建了一种简单的策略来调节 AgNCs 的激发和发射波长。当用一个胞嘧啶替换发射体间隔区中的胸腺嘧啶时，预期的激发和发射变化不会发生。然而，引入的胞嘧啶移动后，DNA 模板会产生与初始模板完全不同的激发和发射波长的 AgNCs，从而产生具有最高荧光强度的近红外（NIR）发射物种的模板。AgNCs 的形成基于 PAGE 中迁移率的改变将 DNA 模板诱导成凝聚的二级结构。在发射体域的间隔区移动一个胞嘧啶的简单策略可以丰富合成各种 DNA/AgNCs 的模板库，在生物成像和探针设计方面具有巨大的潜力。
