State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
J Ultrasound Med. 2021 Sep;40(9):1811-1822. doi: 10.1002/jum.15562. Epub 2020 Nov 11.
To explore the ameliorating effects of low-intensity pulsed ultrasound (LIPUS) on Sprague Dawley rat myelosuppression induced by cell cycle specificity drugs (docetaxel, mitotic phase sensitive; and etoposide, gap 2 phase sensitive).
Rats were respectively administered docetaxel (100 mg/kg) or etoposide (110 mg/kg) by intraperitoneal injection for 4 consecutive days. Then the rats were divided randomly into a LIPUS group and a non-LIPUS group. In the LIPUS group, the right femoral metaphysis of rats was treated by LIPUS (acoustic intensity, 200 mW/cm ; frequency, 0.3 MHz; and duty cycle, 20%) for 20 minutes on 7 consecutive days from day 5. The rats of the non-LIPUS group were treated without ultrasound output. A blood cell count, an enzyme-linked immunosorbent assay, a real-time quantitative polymerase chain reaction, and hematoxylin-eosin staining were applied to detect the results.
Low-intensity pulsed ultrasound significantly promoted the counts of bone marrow nucleated cells, white blood cells, immunoglobulin A (IgA), IgG, granulocyte colony-stimulating factor, stem cell factor, and intercellular cell adhesion molecule 1 and reduced the counts of vascular cell adhesion molecule 1 whether in the docetaxel or etoposide group (P < .05). Low-intensity pulsed ultrasound only increased the expression level of IgM in the docetaxel group but decreased the level of interleukin 6 in the etoposide group (P < .05).
Low-intensity pulsed ultrasound has potential to be a noninvasive treatment for myelosuppression caused by different cell cycle-sensitive chemotherapy drugs.
探讨低强度脉冲超声（LIPUS）对周期特异性药物（多西他赛，有丝分裂期敏感；依托泊苷，间隙 2 期敏感）诱导的 Sprague Dawley 大鼠骨髓抑制的改善作用。
大鼠分别腹腔注射多西他赛（100mg/kg）或依托泊苷（110mg/kg），连续 4 天。然后将大鼠随机分为 LIPUS 组和非 LIPUS 组。在 LIPUS 组中，从第 5 天开始，连续 7 天每天对大鼠右侧股骨干骺端进行 LIPUS（声强 200mW/cm ；频率 0.3MHz；占空比 20%）治疗 20 分钟。非 LIPUS 组大鼠不进行超声输出处理。应用血细胞计数、酶联免疫吸附试验、实时定量聚合酶链反应和苏木精-伊红染色检测结果。
LIPUS 显著促进了骨髓有核细胞、白细胞、免疫球蛋白 A（IgA）、IgG、粒细胞集落刺激因子、干细胞因子和细胞间黏附分子 1 的计数，降低了血管细胞黏附分子 1 的计数，无论在多西他赛组还是依托泊苷组中（P<.05）。LIPUS 仅增加了多西他赛组中 IgM 的表达水平，而降低了依托泊苷组中白细胞介素 6 的水平（P<.05）。
LIPUS 有可能成为一种非侵入性治疗不同细胞周期敏感化疗药物引起的骨髓抑制的方法。
