State Key Laboratory of Ultrasound Engineering in Medicine, cofounded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Collaborative Innovation Center for Minimally Invasive and Noninvasive Medicine, Chongqing Medical University, Chongqing, China.
Department of Physics, University of Vermont, Burlington, Vermont, USA.
J Ultrasound Med. 2019 Oct;38(10):2721-2731. doi: 10.1002/jum.14979. Epub 2019 Mar 5.
This study aimed to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on cyclophosphamide (CTX)-induced rabbit myelosuppression.
Rabbits (n = 90) were randomly divided into a mild myelosuppression group (n = 40), a severe myelosuppression group (n = 40), and a normal control group (group Cu ; n = 10). The mild and severe myelosuppression models were established by daily ear vein injection of 15- and 40-mg/kg CTX for 4 continuous days, respectively. Then they were randomly divided into LIPUS groups (A and B ) and control groups (A and B ). LIPUS was applied once per day for 20 minutes for 7 (A and B ) and 28 (A and B ) days. Physical conditions, mortality, blood cell counts, and bone marrow proliferation were calculated. Erythropoietin interleukin 3, and granulocyte-macrophage colony-stimulating factor levels were measured by an enzyme-linked immunosorbent assay. Flow cytometry was used to detect the granulocyte phagocytosis rate. Hematoxylin-eosin staining was performed to analyze changes of skin and muscle.
Compared with the control group, LIPUS improved the number of peripheral blood cells (P < .05) and bone marrow nucleated cells and reduced the mortality of rabbits with myelosuppression of different degrees. Long-term treatment for 28 days had no effect on the levels of erythropoietin, interleukin 3, and granulocyte-macrophage colony-stimulating factor and granulocyte phagocytosis (P > .05). The parts of the skin where LIPUS was applied did not show any burning marks, and the muscle tissue in the path of LIPUS acoustic channels showed no obvious pathologic changes.
Low-intensity pulsed ultrasound is a safe and effective method to relieve CTX-induced myelosuppression.
本研究旨在探讨低强度脉冲超声（LIPUS）对环磷酰胺（CTX）诱导的兔骨髓抑制的影响。
将 90 只兔子随机分为轻度骨髓抑制组（n=40）、重度骨髓抑制组（n=40）和正常对照组（Cu 组，n=10）。通过每天耳静脉注射 15 和 40mg/kgCTX 连续 4 天建立轻度和重度骨髓抑制模型。然后将它们随机分为 LIPUS 组（A 和 B）和对照组（A 和 B）。LIPUS 每天应用一次，每次 20 分钟，共 7（A 和 B）和 28（A 和 B）天。计算身体状况、死亡率、血细胞计数和骨髓增殖。通过酶联免疫吸附试验测量红细胞生成素、白细胞介素 3 和粒细胞-巨噬细胞集落刺激因子的水平。使用流式细胞术检测粒细胞吞噬率。苏木精-伊红染色分析皮肤和肌肉的变化。
与对照组相比，LIPUS 改善了不同程度骨髓抑制兔的外周血血细胞计数（P<0.05）和骨髓有核细胞数，并降低了死亡率。长期治疗 28 天对红细胞生成素、白细胞介素 3 和粒细胞-巨噬细胞集落刺激因子以及粒细胞吞噬率没有影响（P>0.05）。接受 LIPUS 治疗的皮肤部位没有出现灼伤痕迹，LIPUS 声通道路径中的肌肉组织没有明显的病理变化。
低强度脉冲超声是一种安全有效的缓解环磷酰胺诱导的骨髓抑制的方法。
