Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, 412 96 Gothenburg, Sweden.
Chembiochem. 2021 Mar 2;22(5):807-813. doi: 10.1002/cbic.202000622. Epub 2020 Nov 11.
Exocytosis plays an essential role in the communication between cells in the nervous system. Understanding the regulation of neurotransmitter release during exocytosis and the amount of neurotransmitter content that is stored in vesicles is of importance, as it provides fundamental insights to understand how the brain works and how neurons elicit a certain behavior. In this minireview, we summarize recent progress in amperometric measurements for monitoring exocytosis in single cells and electrochemical cytometry measurements of vesicular neurotransmitter content in individual vesicles. Important steps have increased our understanding of the different mechanisms of exocytosis. Increasing evidence is firmly establishing that partial release is the primary mechanism of release in multiple cell types.
胞吐作用在神经系统细胞间的通讯中起着至关重要的作用。理解胞吐过程中神经递质释放的调节以及囊泡中储存的神经递质含量对于理解大脑如何工作以及神经元如何引发特定行为具有重要意义。在这篇综述中，我们总结了最近在单细胞中监测胞吐作用的安培测量法和单个囊泡中囊泡神经递质含量的电化学细胞测量法的进展。这些重要步骤增加了我们对不同胞吐作用机制的理解。越来越多的证据确凿地表明，部分释放是多种细胞类型释放的主要机制。
