Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.
J Am Chem Soc. 2020 Mar 4;142(9):4093-4097. doi: 10.1021/jacs.9b13221. Epub 2020 Feb 24.
We have developed the means to simultaneously measure the physical size and count catecholamine molecules in individual nanometer transmitter vesicles. This is done by combining resistive pulse (RP) measurements in a nanopore pipet and vesicle impact electrochemical cytometry (VIEC) at an electrode as the vesicle exits the nanopore. Analysis of freshly isolated bovine adrenal vesicles shows that the size and internal catecholamine concentration of vesicles varies with the occurrence of a dense core inside the vesicles. These results might benefit the understanding about the vesicles maturation, especially involving the "sorting by retention" process and concentration increase of intravesicular catecholamine. The methodology is applicable to understanding soft nanoparticle collisions on electrodes, vesicles in exocytosis and phagocytosis, intracellular vesicle transport, and analysis of electroactive drugs in exosomes.
我们已经开发出了一种方法，可以同时测量单个纳米发射器囊泡的物理尺寸和儿茶酚胺分子的数量。这是通过在纳米孔移液器中进行电阻脉冲（RP）测量，并在囊泡离开纳米孔时在电极上进行囊泡撞击电化学细胞计数（VIEC）来实现的。对新分离的牛肾上腺囊泡的分析表明，囊泡的大小和内部儿茶酚胺浓度随囊泡内出现致密核心而变化。这些结果可能有助于了解囊泡的成熟过程，特别是涉及“通过保留进行分拣”过程和囊泡内儿茶酚胺浓度的增加。该方法适用于理解电极上的软纳米颗粒碰撞、胞吐和胞吞中的囊泡、细胞内囊泡运输以及外泌体中电化学药物的分析。
