Graduada em Ciências Biológicas pela Universidade Federal do Triângulo Mineiro/UFTM, Uberaba, MG, Brasil.
Professora do Instituto de Ciências Biológicas e Naturais (ICBN) da UFTM, Uberaba, MG, Brasil.
Rev Assoc Med Bras (1992). 2020 Oct;66(10):1396-1401. doi: 10.1590/1806-9282.66.10.1396.
The relationship between the clinicopathological and sociodemographics characteristics of acral melanomas diagnosed at BACKGROUND: This study aimed to investigate the frequency of VEGF gene insertion (I) / deletion (D) polymorphism (rs35569394) in patients with Polycystic Ovarian Syndrome (PCOS) and to compare with a control population to verify its association with the pathology.
206 women participated in this study, 103 with PCOS (group of patients) and 103 without the disease (control group). After extraction of genomic DNA from the samples, molecular analysis was performed by Polymerase Chain Reaction (PCR) and electrophoresis in polycrylamide. Descriptive analysis, univariate analysis and logistic regression model were used. Results were presented in odds ratio (OR) and 95% confidence interval (95% CI), considering the significance of p <0.05.
There were no statistical differences between patients and controls for allele frequencies (χ2 = 1.16, p = 0.56). The genotypic frequency distribution was in Hardy Weinberg equilibrium for the patients (χ2 = 2.42; p <0.05), but not for the control group (χ2 = 7.26; p <0.05). Regarding risk factors for the syndrome, a history of familial PCOS is more frequent among women with the syndrome.
In the present study, there is no association between VEGF gene I / D polymorphism and PCOS.
研究肢端黑素瘤的临床病理和社会人口统计学特征与 BACKGROUND：本研究旨在调查血管内皮生长因子（VEGF）基因插入（I）/缺失（D）多态性（rs35569394）在多囊卵巢综合征（PCOS）患者中的频率，并与对照组进行比较，以验证其与病理学的相关性。
206 名女性参与了这项研究，其中 103 名患有 PCOS（患者组），103 名没有该病（对照组）。从样本中提取基因组 DNA 后，通过聚合酶链反应（PCR）和聚丙烯酰胺电泳进行分子分析。采用描述性分析、单变量分析和逻辑回归模型。结果以比值比（OR）和 95%置信区间（95%CI）表示，p<0.05 为有统计学意义。
患者和对照组之间等位基因频率无统计学差异（χ2=1.16，p=0.56）。患者的基因型频率分布符合 Hardy-Weinberg 平衡（χ2=2.42；p<0.05），但对照组不符合（χ2=7.26；p<0.05）。关于该综合征的危险因素，有家族性 PCOS 病史的女性更易患该综合征。
本研究中，VEGF 基因 I/D 多态性与 PCOS 之间无关联。
