Fernández R A, Ciccarelli A S, Arenas G N, Giménez D F
Cátedra de Microbiología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
Rev Argent Microbiol. 1986;18(1):29-31.
In December 1982 an outbreak of foodborne botulism presumably produced by the ingestion of home-made pickled trout occurred in San Rafael, province of Mendoza, Argentina. The toxin detected in blood serum and feces samples of the sole affected patient was preliminarily typed as a plain type A. A strain of Clostridium botulinum was isolated from feces which, after culture by the dialysis method, produced 1 x 10(7) LD50/mouse per ml. Quantitative neutralization tests carried out at different levels of toxin concentration, showed that this toxin consists of a major type A antigenic component (about 99% of the complex) and a minor type F component, defining its identity as a subtype Af. A rabbit antiserum obtained from this toxin neutralizes A and F reference toxins. Despite the antitoxic and supportive treatment, the patient died as a consequence of the poisoning. The polyvalent antiserum administered contained A, B and E antitoxins. Death could be due to the lethal effect of the A fraction, the F fraction or to a combined effect of both toxic components of the toxin. This is the first detection of an outbreak produced by C. botulinum subtype Af, serotype described in Argentina fifteen years ago, and not detected until now in other parts of the world.(ABSTRACT TRUNCATED AT 250 WORDS)
1982年12月，阿根廷门多萨省圣拉斐尔发生了一起食源性肉毒中毒事件，推测是由于食用了自制腌制鳟鱼所致。在唯一一名受影响患者的血清和粪便样本中检测到的毒素初步鉴定为纯A型。从粪便中分离出一株肉毒杆菌，经透析法培养后，每毫升产生1×10⁷半数致死量/小鼠。在不同毒素浓度水平下进行的定量中和试验表明，这种毒素由一种主要的A型抗原成分（约占复合物的99%）和一种次要的F型成分组成，确定其为Af亚型。从这种毒素获得的兔抗血清可中和A和F参考毒素。尽管进行了抗毒素和支持性治疗，患者仍因中毒死亡。所给予的多价抗血清含有A、B和E抗毒素。死亡可能是由于A组分、F组分的致死作用，或毒素两种毒性成分的联合作用。这是首次检测到由肉毒杆菌Af亚型引起的疫情，该血清型于15年前在阿根廷被描述，至今在世界其他地区尚未被检测到。（摘要截短至250字）
