Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford School of Medicine, Stanford, CA 94305, USA.
Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, CA 94305, USA.
Cell Rep. 2020 Nov 10;33(6):108356. doi: 10.1016/j.celrep.2020.108356.
Fibroblast heterogeneity has been shown within the unwounded mouse dorsal dermis, with fibroblast subpopulations being identified according to anatomical location and embryonic lineage. Using lineage tracing, we demonstrate that paired related homeobox 1 (Prrx1)-expressing fibroblasts are responsible for acute and chronic fibroses in the ventral dermis. Single-cell transcriptomics further corroborated the inherent fibrotic characteristics of Prrx1 fibroblasts during wound repair. In summary, we identify and characterize a fibroblast subpopulation in the mouse ventral dermis with intrinsic scar-forming potential.
成纤维细胞异质性已在未受伤的小鼠背部真皮中显现，根据解剖位置和胚胎谱系鉴定出成纤维细胞亚群。通过谱系追踪，我们证明表达配对相关同源盒 1（Prrx1）的成纤维细胞负责腹侧真皮的急性和慢性纤维化。单细胞转录组学进一步证实了 Prrx1 成纤维细胞在伤口修复过程中的固有纤维化特征。总之，我们在小鼠腹侧真皮中鉴定和表征了具有内在瘢痕形成潜力的成纤维细胞亚群。
