Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Department of Biological Production, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Int Arch Allergy Immunol. 2021;182(4):292-300. doi: 10.1159/000511239. Epub 2020 Nov 11.
Recently, the relationship between antigen contact via skin (skin sensitization) and the development of food allergies has gained increasing attention. However, few studies have examined the effects of skin sensitization on healthy skin.
To examine the effect of sensitization in healthy skin on IgE and cytokine production during food allergy development.
The effect of skin sensitization on food allergy was evaluated using DO11.10 mice whose T cells express ovalbumin (OVA)-specific T-cell receptors. OVA was applied to the back skin of mice dehaired by various methods, and then food allergy was induced by providing them with an OVA-containing diet. OVA-specific IgE production in the sera and decreases in body temperature due to anaphylactic reaction were measured as indicators of food allergy. In addition, IL-4 production and proliferation of splenocytes were measured in mice with food allergy after skin sensitization.
Skin sensitization in healthy skin increased IgE production and exacerbated anaphylactic symptoms induced by ingesting the antigen. Moreover, skin sensitization enhanced IL-4 production from splenocytes during the onset of food allergy. In contrast, oral tolerance was induced even after establishing skin sensitization.
Skin sensitization temporarily exacerbated food allergy by enhancing systemic Th2 responses. These findings will help identify the mechanisms involved in food allergy and help develop treatments.
最近，通过皮肤接触抗原（皮肤致敏）与食物过敏发展之间的关系引起了越来越多的关注。然而，很少有研究检查皮肤致敏对健康皮肤的影响。
研究健康皮肤中的致敏作用对食物过敏发展过程中 IgE 和细胞因子产生的影响。
使用表达卵清蛋白（OVA）特异性 T 细胞受体的 DO11.10 小鼠评估皮肤致敏对食物过敏的影响。通过各种方法去除小鼠背部毛发，将 OVA 应用于皮肤，然后用含 OVA 的饮食诱导食物过敏。通过测量血清中 OVA 特异性 IgE 的产生和由于过敏反应引起的体温下降来评估食物过敏。此外，在皮肤致敏后测量食物过敏小鼠的脾细胞中 IL-4 产生和增殖。
健康皮肤中的致敏作用增加了 IgE 的产生，并加重了摄入抗原引起的过敏症状。此外，皮肤致敏增强了食物过敏发作时脾细胞中 IL-4 的产生。相比之下，即使在建立皮肤致敏后，也能诱导口服耐受。
皮肤致敏通过增强全身 Th2 反应暂时加重了食物过敏。这些发现将有助于确定食物过敏涉及的机制，并有助于开发治疗方法。
