Department of Orthopedic, 74569China-Japan Union Hospital of Jilin University, Changchun, Jilin, People's Republic of China.
Department of Internal Neurology, 154454The Second Hospital of Jilin University, Changchun, Jilin, People's Republic of China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820973278. doi: 10.1177/1533033820973278.
Osteosarcoma (OS) is a cancerous tumor in a bone. We aimed to identify the critical genes involved in OS progression, and then try to elucidate the molecular mechanisms of this disease. The microarray data of GSE32395 was used for the present study. We analyzed differentially expressed genes (DEGs) in OS cells compared with control group by Student's t-test. The significant enriched gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathways were analyzed for upregulated genes and downregulated genes, respectively. In addition, a protein-protein interaction (PPI) network was constructed. GO and KEGG enrichment analyses were conducted for genes in the PPI network. In total, 183 DEGs, including 100 upregulated DEGs and 83 downregulated DEGs were screened. The upregulated DEGs were significantly enriched in 2 KEGG pathways, such as "Glycosaminoglycan biosynthesis-chondroitin sulfate" and the downregulated DEGs were significantly enriched in 12 pathways, including "cell adhesion molecules," "pentose phosphate pathway" and "allograft rejection." GO enrichment analysis indicated that the upregulated DEGs were significantly involved in biological process, such as "multicellular organismal metabolic process" and "limb morphogenesis," while the downregulated DEGs were significantly enriched in biological process, such as "Positive regulation of pathway-restricted SMAD protein phosphorylation." The PPI network included 84 interactions and 51 nodes. The "glycosaminoglycan biosynthesis-chondroitin sulfate pathway," "microtubule motor activityfunction," and "regulation of mitosis process" were significantly enriched by genes in PPI network. In particular, CENPE, PRC1, TTK, and PLK4 had higher degrees in the PPI network. The interactions between TTK and PLK4 as well as CENPE and PRC1 may involve in the OS development. These 4 genes might be possible biomarkers for the treatment and diagnosis of OS.
骨肉瘤（OS）是一种骨内的癌性肿瘤。我们旨在鉴定参与 OS 进展的关键基因，并尝试阐明这种疾病的分子机制。本研究使用了 GSE32395 的微阵列数据。我们通过学生 t 检验分析了 OS 细胞与对照组之间差异表达的基因（DEGs）。分别对上调基因和下调基因进行了显著富集的基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析。此外，还构建了蛋白质-蛋白质相互作用（PPI）网络。对 PPI 网络中的基因进行了 GO 和 KEGG 富集分析。总共筛选出 183 个 DEGs，包括 100 个上调 DEGs 和 83 个下调 DEGs。上调的 DEGs 显著富集在 2 个 KEGG 通路中，如“糖胺聚糖生物合成-硫酸软骨素”，而下调的 DEGs 则显著富集在 12 个通路中，包括“细胞黏附分子”、“戊糖磷酸途径”和“同种异体移植排斥”。GO 富集分析表明，上调的 DEGs 主要参与生物过程，如“多细胞生物代谢过程”和“肢体形态发生”，而下调的 DEGs 则主要富集在生物过程中，如“途径受限 SMAD 蛋白磷酸化的正调控”。PPI 网络包括 84 个相互作用和 51 个节点。PPI 网络中的基因显著富集在“糖胺聚糖生物合成-硫酸软骨素途径”、“微管马达活性功能”和“有丝分裂过程的调节”中。特别是，PPI 网络中 CENPE、PRC1、TTK 和 PLK4 的度值较高。TTK 和 PLK4 之间以及 CENPE 和 PRC1 之间的相互作用可能涉及 OS 的发展。这 4 个基因可能是 OS 治疗和诊断的潜在生物标志物。
