Ugwu I C, Lee-Ching L, Ugwu C C, Okoye J O A, Chah K F
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.
Department of Bioprocess Technology, School of Industrial Technology, University Sains Malaysia, Penang, Malaysia.
Iran J Vet Res. 2020 Summer;21(3):180-187.
Avian pathogenic (APEC) strains have been associated with various disease conditions in avian species due to virulence attributes associated with the organism.
This study was carried out to determine the pathogenic characteristics and virulence encoding genes found in strains associated with colibacillosis in chickens.
Fifty-two stock cultures of strains isolated from chickens diagnosed of colibacillosis were tested for their ability to produce haemolysis on blood agar and take up Congo red dye. Molecular characterization was carried out by polymerase chain reaction (PCR) amplification of virulence encoding genes associated with APEC.
Eleven (22%) and 41 (71%) were positive for haemolysis on 5% sheep red blood agar and Congo red agar, respectively. Nine virulence-associated genes were detected as follows: (96%), (52%), (48%), (33%), (21%), (15%), (10%), (2%), and (2%)
The APEC strains exhibited virulence properties and harbored virulence encoding genes which could be a threat to the poultry population and public health. The putative virulence genes were diverse and different in almost all isolate implying that pathogenesis was multi-factorial and the infection was multi-faceted which could be a source of concern in the detection and control of APEC infections.
禽致病性大肠杆菌（APEC）菌株因其与该生物体相关的毒力特性，已与禽类的各种疾病状况有关。
本研究旨在确定从患有大肠杆菌病的鸡中分离出的菌株的致病特征和毒力编码基因。
对从诊断患有大肠杆菌病的鸡中分离出的52株菌株进行库存培养，检测它们在血琼脂上产生溶血和摄取刚果红染料的能力。通过聚合酶链反应（PCR）扩增与APEC相关的毒力编码基因进行分子特征分析。
分别有11株（22%）和41株（71%）在5%绵羊红细胞血琼脂和刚果红琼脂上溶血呈阳性。检测到9种与毒力相关的基因如下：（96%）、（52%）、（48%）、（33%）、（21%）、（15%）、（10%）、（2%）和（2%）
APEC菌株表现出毒力特性并携带毒力编码基因，这可能对家禽群体和公共卫生构成威胁。几乎所有分离株中的假定毒力基因都是多样且不同的，这意味着发病机制是多因素的，感染是多方面的，这可能是APEC感染检测和控制中令人担忧的一个问题。
