assessment of pathogenicity and virulence encoding gene profiles of avian pathogenic strains associated with colibacillosis in chickens.

BACKGROUND

Avian pathogenic (APEC) strains have been associated with various disease conditions in avian species due to virulence attributes associated with the organism.

AIMS

This study was carried out to determine the pathogenic characteristics and virulence encoding genes found in strains associated with colibacillosis in chickens.

METHODS

Fifty-two stock cultures of strains isolated from chickens diagnosed of colibacillosis were tested for their ability to produce haemolysis on blood agar and take up Congo red dye. Molecular characterization was carried out by polymerase chain reaction (PCR) amplification of virulence encoding genes associated with APEC.

RESULTS

Eleven (22%) and 41 (71%) were positive for haemolysis on 5% sheep red blood agar and Congo red agar, respectively. Nine virulence-associated genes were detected as follows: (96%), (52%), (48%), (33%), (21%), (15%), (10%), (2%), and (2%)

CONCLUSION

The APEC strains exhibited virulence properties and harbored virulence encoding genes which could be a threat to the poultry population and public health. The putative virulence genes were diverse and different in almost all isolate implying that pathogenesis was multi-factorial and the infection was multi-faceted which could be a source of concern in the detection and control of APEC infections.