Laopiem Sudtisa, Witoonsatian Kriangkrai, Kulprasetsri Sittinee, Panomwan Pun, Pathomchai-Umporn Chutima, Kamtae Raktipon, Jirawattanapong Pichai, Songserm Thaweesak, Sinwat Nuananong
Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Kamphaengsean Campus, Nakorn Pathom, 73140, Thailand.
Kamphaeng Saen Veterinary Diagnostic Center, Faculty of Veterinary Medicine, Kasetsart University, Kamphaengsean campus, Nakorn Pathom, 73140, Thailand.
BMC Vet Res. 2025 Mar 8;21(1):160. doi: 10.1186/s12917-025-04626-x.
Multidrug resistance in Escherichia coli has a significant global impact on poultry production. This study aimed to determine the phenotypic and genotypic backgrounds of antimicrobial resistance (AMR) and virulence gene profiles of E. coli strains isolated from diseased and healthy broilers. A total of 211 E. coli isolates were recovered from diseased (n = 110) and healthy broilers (n = 101). All the isolates were subjected to antimicrobial susceptibility testing. A PCR-based technique was applied to screen AMR genes, virulence genes and analyze phylogenetic groups.
Phylogenetic groups B1 and D were the most prevalent for E. coli isolated from diseased and healthy birds. Among virulence genes, the detection rates of cva/cvi, iutA, iucD, iroN, iss and ompT were considerably greater in E.coli strains from diseased birds than in healthy birds. The virulence gene pattern of hlyF-iutA-iucD-iroN-iss-ompT (16.4%) was frequently observed in E.coli isolated from diseased birds, whereas approximately 22.8% of E.coli from healthy birds did not carry any virulence genes. Analysis of AMR profiles revealed that 58.3% of E.coli were resistant to multiple classes of antibiotics, and 96.7% carried at least one antibiotic resistance gene AMR genes.
The findings of this study demonstrate the variable distribution of phylogenetic groups and virulence genes. E.coli strains isolated from broilers had multidrug resistance profiles. The study emphasizes the need for continuous monitoring of AMR emergence in E. coli from broilers. This monitoring allows for early detection and implementation of strategies to control the spread of resistant strains.
大肠杆菌中的多重耐药性对全球家禽生产有重大影响。本研究旨在确定从患病和健康肉鸡中分离出的大肠杆菌菌株的抗菌药物耐药性（AMR）的表型和基因型背景以及毒力基因谱。总共从患病（n = 110）和健康肉鸡（n = 101）中分离出211株大肠杆菌。所有分离株均进行了抗菌药物敏感性测试。应用基于PCR的技术筛选AMR基因、毒力基因并分析系统发育群。
系统发育群B1和D在从患病和健康禽类中分离出的大肠杆菌中最为普遍。在毒力基因中，患病禽类的大肠杆菌菌株中cva/cvi、iutA、iucD、iroN、iss和ompT的检出率明显高于健康禽类。hlyF-iutA-iucD-iroN-iss-ompT（16.4%）的毒力基因模式在从患病禽类中分离出的大肠杆菌中经常观察到，而来自健康禽类的大肠杆菌中约22.8%不携带任何毒力基因。AMR谱分析显示，58.3%的大肠杆菌对多种类抗生素耐药，96.7%携带至少一种抗生素耐药基因AMR基因。
本研究结果表明系统发育群和毒力基因的分布存在差异。从肉鸡中分离出的大肠杆菌菌株具有多重耐药谱。该研究强调需要持续监测肉鸡大肠杆菌中AMR的出现情况。这种监测有助于早期发现并实施控制耐药菌株传播的策略。
