Xiao Jimei, Song Jingyan, Sa Yuanhong, Yuan Lihua, Guo Jiayin, Sun Zhengao
The First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Evid Based Complement Alternat Med. 2020 Oct 31;2020:5183017. doi: 10.1155/2020/5183017. eCollection 2020.
Diminished ovarian reserve (DOR) is the weakening of ovarian oocyte production and quality. It will further become premature ovarian failure without timely cure. However, disease pathology and diagnostic markers are still incompletely understood. Liu-Wei-Di-Huang (LWDH) pill, a traditional Chinese medicine formula, is commonly used in the treatment of DOR in China. To explore the mechanism of the effect of LWDH on in vitro fertilization (IVF) outcomes in patients with DOR, a pseudotargeted metabolomics study combined with multivariate data processing strategy was carried out. A liquid chromatography tandem mass spectrometry-based metabolomics approach was applied to characterize metabolic biomarker candidates. Multiple pattern recognition was used to determine groups and confirm important variables. A total of 21 potential biomarkers were characterized, and related metabolic pathways were identified. The study displayed that the established pseudotargeted metabolomics strategy is a powerful approach for investigating the mechanism of DOR and LWDH. In addition, the approach may highlight biomarkers and metabolic pathways and can capture subtle metabolite changes from headache, which may lead to an improved mechanism understanding of DOR diseases and LWDH treatment.
卵巢储备功能减退（DOR）是指卵巢卵母细胞产生能力和质量的减弱。若不及时治疗，它会进一步发展为卵巢早衰。然而，疾病病理和诊断标志物仍未被完全了解。六味地黄丸（LWDH），一种中药配方，在中国常用于治疗DOR。为了探究LWDH对DOR患者体外受精（IVF）结局影响的机制，开展了一项结合多变量数据处理策略的伪靶向代谢组学研究。采用基于液相色谱串联质谱的代谢组学方法来表征潜在的代谢生物标志物。运用多种模式识别来确定分组并确认重要变量。共鉴定出21种潜在生物标志物，并确定了相关代谢途径。该研究表明，所建立的伪靶向代谢组学策略是研究DOR和LWDH机制的有力方法。此外，该方法可能会突出生物标志物和代谢途径，并能从细微之处捕捉代谢物变化，这可能有助于提高对DOR疾病和LWDH治疗机制的理解。
