Park Youngjin, Zhang Qirui, Wiegertjes Geert F, Fernandes Jorge M O, Kiron Viswanath
Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
Division of Clinical Genetics, Lund University, Lund, Sweden.
Front Cell Dev Biol. 2020 Oct 15;8:580848. doi: 10.3389/fcell.2020.580848. eCollection 2020.
Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of BioParticles. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells.
与哺乳动物相比，我们对鱼类肠道免疫系统的了解相当有限。对于鱼类肠道中的免疫细胞，包括吞噬细胞，我们所知甚少。因此，我们采用成像流式细胞术和RNA测序技术，研究了从健康大西洋鲑鱼中分离出的贴壁细胞。将远端肠道的贴壁细胞（贴壁肠道细胞，或AIC）与头肾的贴壁细胞（贴壁肾细胞，或AKC）的吞噬活性和选定基因表达进行了比较。基于BioParticles的摄取评估了这两种细胞类型的吞噬活性。AIC显示出吞噬能力，但与AKC相比，吞噬细胞的形态不同。转录组分析表明，AIC表达了与巨噬细胞、T细胞和内皮细胞相关的基因。对选定基因的热图分析表明，来自两个器官的贴壁细胞中巨噬细胞相关基因的表达明显更高。我们认为贴壁肠道细胞具有吞噬特性，且通常与巨噬细胞相关的基因表达较高。我们设想了未来对富集的贴壁肠道细胞群体进行研究的可能性。
