Zhao Ya-Bin, Li Wei, Zhang Qin, Yin Yan, Yang Chuan-Jia, Xu Wen-Xiang, Kang Jian, Qi Rui-Qun, Hou Gang
Department of Respiratory Medicine, First Hospital of China Medical University, Shenyang, China.
Department of Respiratory Medicine, The Third People's Hospital of Hubei Province, Wuhan, China.
Front Med (Lausanne). 2020 Oct 16;7:527433. doi: 10.3389/fmed.2020.527433. eCollection 2020.
Sarcoidosis and tuberculosis share similarities in clinical manifestations and histopathological features. We aimed to identify the microRNA (miRNA) profiles of the lymph nodes of individuals with sarcoidosis and of those with tuberculous lymphadenitis to investigate the value of miRNAs in the differential diagnosis of sarcoidosis and tuberculous lymphadenitis. The miRNA profiles of the lymph nodes of individuals with sarcoidosis, those with tuberculous lymphadenitis (TBLN) and controls were detected by miRNA microarray analysis in the age- and sex-matched development group of the controls ( = 3), patients with TBLN ( = 3) and patients with sarcoidosis ( = 3), and the results were validated by quantitative real-time polymerase chain reaction in the validation group of the controls ( = 30), TBLN ( = 30) and patients with sarcoidosis ( = 31). The relationship between miRNA expression and the clinical parameters of sarcoidosis was analyzed. miR-145, miR-185-5p, miR-301, miR-425-5P, miR-449b and miR-885-5P were differentially expressed between individuals with sarcoidosis and controls ( < 0.0001, < 0.0001, = 0.0008, = 0.0002, = 0.0018, and < 0.0001, respectively), and the same six miRNAs were differentially expressed between individuals with tuberculous lymphadenitis and controls ( = 0.0002, = 0.0004, = 0.0238, = 0.0006, = 0.0149, and = 0.0045, respectively). miR-185-5p was differentially expressed between individuals with tuberculous lymphadenitis and those with sarcoidosis ( = 0.0101). The area under the receiver operating characteristic curve calculated for miR-185-5p was 0.6860, and the sensitivity and specificity of miR-185-5p for the differential diagnosis of sarcoidosis from TBLN were 61 and 80%, respectively. The levels of miR-145, miR-301, miR-425-5P, and miR-885-5P were positively correlated with CD4+/CD8+ T lymphocytes in bronchoalveolar lavage fluid. miRNAs in lymph nodes show similar expression patterns between individuals with sarcoidosis and those with tuberculous lymphadenitis, which were experimentally selected. miR-185-5p in the lymph nodes can be used as an auxiliary marker for the differential diagnosis of sarcoidosis and tuberculous lymphadenitis.
结节病和结核病在临床表现和组织病理学特征上有相似之处。我们旨在鉴定结节病患者和结核性淋巴结炎患者淋巴结中的微小RNA（miRNA）谱，以研究miRNA在结节病和结核性淋巴结炎鉴别诊断中的价值。通过miRNA微阵列分析检测了对照组（n = 3）、结核性淋巴结炎患者（n = 3）和结节病患者（n = 3）年龄和性别匹配的发育组中结节病患者、结核性淋巴结炎（TBLN）患者和对照组淋巴结的miRNA谱，并在对照组（n = 30）、TBLN（n = 30）和结节病患者（n = 31）的验证组中通过定量实时聚合酶链反应对结果进行了验证。分析了miRNA表达与结节病临床参数之间的关系。miR-145、miR-185-5p、miR-301、miR-425-5P、miR-449b和miR-885-5P在结节病患者和对照组之间差异表达（分别为P < 0.0001、P < 0.0001、P = 0.0008、P = 0.0002、P = 0.0018和P < 0.0001），并且相同的六个miRNA在结核性淋巴结炎患者和对照组之间差异表达（分别为P = 0.0002、P = 0.0004、P = 0.0238、P = 0.0006、P = 0.0149和P = 0.0045）。miR-185-5p在结核性淋巴结炎患者和结节病患者之间差异表达（P = 0.0101）。为miR-185-5p计算的受试者工作特征曲线下面积为0.6860，miR-185-5p对结节病与TBLN鉴别诊断的敏感性和特异性分别为61%和80%。miR-145、miR-301、miR-425-5P和miR-885-5P的水平与支气管肺泡灌洗液中的CD4+/CD8+ T淋巴细胞呈正相关。实验选择的结节病患者和结核性淋巴结炎患者淋巴结中的miRNA显示出相似的表达模式。淋巴结中的miR-185-5p可作为结节病和结核性淋巴结炎鉴别诊断的辅助标志物。
