大鼠创伤性脑损伤后血小板衍生生长因子-D 及其受体-β 的动态表达。

Dynamic Expression of Platelet-Derived Growth Factor-D and Phosphorylated Platelet-Derived Growth Factor Receptor-Β after Traumatic Brain Injury in Rats.

出版信息

Clin Lab. 2020 Nov 1;66(11). doi: 10.7754/Clin.Lab.2020.200328.

DOI:10.7754/Clin.Lab.2020.200328

Abstract

BACKGROUND

We explored the dynamic expression of platelet-derived growth factor-D (PDGF-D) and phosphorylated platelet-derived growth factor receptor-β (p-PDGFR-β) after traumatic brain injury in rats to provide theoretical basis for selecting therapeutic target and intervention time after traumatic brain injury.

METHODS

This study prepared the weight drop/impact acceleration-induced traumatic brain injury (TBI) model in rats, including sham group and TBI groups at different observation time points (6 hours, 12 hours, 24 hours, 3 days, and 7 days after TBI). The dynamic expression of PDGF-D and p-PDGFR-β after TBI were detected by western blot and immunofluorescence staining.

RESULTS

The expression level of PDGF-D and p-PDGFR-β after TBI was detected by western blot. The PDGF-D level was increased (p < 0.05) 6 hours after TBI and remained at the high level until 3 days after TBI (p < 0.05). The p-PDGFR-β level was increased (p < 0.05) 12 hours after TBI and remained at the high level until 3 days after TBI (p < 0.05). PDGF-D and p-PDGFR-β in brain tissues were found by immunofluorescence in the perihematoma area 24 hours after TBI.

CONCLUSIONS

This study revealed the expression phenomenon of PDGF-D and p-PDGFR-β after TBI in rats, suggesting that PDGF-D participates in the process of secondary brain injury after TBI through specific binding with PDGFR-β, which provides a theoretical basis for further research on selecting therapeutic targets and intervention times after TBI.

摘要

背景

本研究旨在探讨血小板衍生生长因子-D（PDGF-D）及其磷酸化血小板衍生生长因子受体-β（p-PDGFR-β）在创伤性脑损伤（TBI）大鼠中的动态表达，为选择 TBI 后治疗靶点和干预时间提供理论依据。

方法

本研究采用重物坠落/加速度撞击法制备大鼠 TBI 模型，包括假手术组和 TBI 组，在伤后不同时间点（6 h、12 h、24 h、3 d 和 7 d）观察。采用 Western blot 和免疫荧光染色法检测 TBI 后 PDGF-D 和 p-PDGFR-β 的动态表达。

结果

Western blot 检测发现 TBI 后 PDGF-D 和 p-PDGFR-β 的表达水平升高，PDGF-D 在伤后 6 h 开始升高（p<0.05），并持续至伤后 3 d（p<0.05）；p-PDGFR-β 在伤后 12 h 开始升高（p<0.05），并持续至伤后 3 d（p<0.05）。伤后 24 h 在血肿周围组织免疫荧光染色发现 PDGF-D 和 p-PDGFR-β。