空气传播真菌通过茉莉酸和水杨酸信号诱导拟南芥中花色素苷的生物合成。
Airborne fungus-induced biosynthesis of anthocyanins in Arabidopsis thaliana via jasmonic acid and salicylic acid signaling.
机构信息
School of Life Science, Anhui Agricultural University, Hefei 230036, China; State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China.
School of Life Science, Anhui Agricultural University, Hefei 230036, China; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
出版信息
Plant Sci. 2020 Nov;300:110635. doi: 10.1016/j.plantsci.2020.110635. Epub 2020 Aug 14.
Anthocyanins are plant-specific pigments, the biosynthesis of which is stimulated by pathogen infection in several plant species. A. thaliana seedlings injected with airborne fungi can accumulate a high content of anthocyanins. The mechanism involved in fungus-induced anthocyanin accumulation in plants has not been fully described. In this study, the fungus Penicillium corylophilum (P. corylophilum), isolated from an Arabidopsis culture chamber, triggered jasmonic acid (JA), salicylic acid (SA), and anthocyanin accumulation in A. thaliana. Inhibitors of JA and SA biosynthesis suppressed the anthocyanin accumulation induced by P. corylophilum. The anthocyanin content was minimal in both the null mutant of JA-receptor coi1 and the null mutant of SA-receptor npr1 under P. corylophilum stimulation. The results indicate that JA and SA signaling mediated fungus-induced anthocyanin biosynthesis in A. thaliana. P. corylophilum led to different levels of anthocyanin generation in null mutants for MYB75, bHLH, EGL3, and GL3 transcription factors and WD40 protein, demonstrating that multiple MYB-bHLH-WD40 transcription factor complexes participated in fungus-induced anthocyanin accumulation in A. thaliana. The present study will help further elucidate the mechanism of plant resistance to pathogen infection.
花色苷是植物特有的色素，其生物合成在几种植物物种受到病原体感染时会受到刺激。向拟南芥幼苗注射空气传播的真菌可以积累高含量的花色苷。真菌诱导植物中花色苷积累的机制尚未完全描述。在这项研究中，从拟南芥培养室中分离出的青霉菌（Penicillium corylophilum）（P. corylophilum）触发了拟南芥中的茉莉酸（JA）、水杨酸（SA）和花色苷的积累。JA 和 SA 生物合成的抑制剂抑制了 P. corylophilum 诱导的花色苷积累。在 P. corylophilum 刺激下，JA 受体 coi1 的 null 突变体和 SA 受体 npr1 的 null 突变体中的花色苷含量均最小。结果表明，JA 和 SA 信号转导介导了真菌诱导的拟南芥花色苷生物合成。青霉菌导致 MYB75、bHLH、EGL3 和 GL3 转录因子和 WD40 蛋白的 null 突变体产生不同水平的花色苷生成，表明多个 MYB-bHLH-WD40 转录因子复合物参与了真菌诱导的拟南芥花色苷积累。本研究将有助于进一步阐明植物对病原体感染的抗性机制。
Zotero 插件