[用于进行免疫酶分析的聚苯乙烯基固相载体适用性标准的选择]

[Choice of the suitability criteria of polystyrene-based solid-phase carriers for performing immunoenzyme analyses].

作者信息

Shakhanina K L, Sokolenko A A, Pavlova I P, Evseeva L F, Umnova N S

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1987 Sep(9):8-11.

Abstract

The authors discuss a tentative approach to the choice of criteria indicating the optimal suitability of different solid-phase carriers made of polystyrene for use in the enzyme immunoassay (EIA), viz. the dependence of specificity, sensitivity, reproducibility and reliability of EIA results on the adsorption properties, transparency expressed in percent and transparency variations of the plates under test. The evaluation of the carriers by four parameters is proposed with the use of assay plates manufactured by Nunc A/S (Denmark) for control. To ensure the objective evaluation of the suitability of polystyrene plates for use in EIA, the choice of uniform criteria is necessary.

摘要

作者讨论了一种初步方法，用于选择表明由聚苯乙烯制成的不同固相载体在酶免疫测定（EIA）中最佳适用性的标准，即EIA结果的特异性、灵敏度、重现性和可靠性对吸附特性、被测板以百分比表示的透明度以及透明度变化的依赖性。建议使用丹麦Nunc A/S公司生产的测定板作为对照，通过四个参数对载体进行评估。为确保对聚苯乙烯板在EIA中适用性的客观评估，需要选择统一的标准。