Wang Y F, He K, Guo W, Wang H F, Zhang D H, Gong M Q, Ji M J, Chen L
School of Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, China.
School of Medical Imaging, Nanjing Medical University, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020 Oct 16;32(5):517-521. doi: 10.16250/j.32.1374.2019197.
To investigate the genes involved in -induced cytoplasmic incompatibility among three natural populations of in eastern China, so as to provide insights into the development of preventive and control measures for mosquito-borne diseases based on .
The cytoplasmic incompatibility was tested among three natural populations of collected from Nanjing and Wuxi of Jiangsu Province and Tangkou of Shandong Province using reciprocal crosses. a infection was detected in using a PCR assay, and the expression of and genes was quantified using a fluorescent quantitative real-time PCR (qPCR) assay.
Bidirectional compatibility was found between the natural populations of collected from Nanjing and Wuxi of Jiangsu Province ( = 0.57 and 0.15, both values > 0.05), while bidirectional incompatibility was seen between the natural populations of collected from Tangkou of Shandong Province and Wuxi of Jiangsu Province ( = 63.81 and 43.51, both values < 0.01), and between the natural populations of collected from Nanjing of Jiangsu Province and Tangkou of Shandong Province ( = 39.62 and 43.12, both values < 0.01). gene was amplified in all three natural populations of , and qPCR assay detected no significant difference in the gene expression among the three natural populations of ( = 2.15, > 0.05). In addition, there was no significant difference in the gene expression between the natural populations of collected from Tangkou of Shandong Province and Nanjing of Jiangsu Province ( = 8.42, < 0.05) or between the natural populations of collected from Tangkou of Shandong Province and Wuxi of Jiangsu Province ( = 7.84, < 0.05); however, there was a significant difference detected in the gene expression between the natural populations of collected from Nanjing and Wuxi of Jiangsu Province ( = 0.40, > 0.05).
Different numbers are detected in natural populations of collected from Nanjing and Wuxi of Jiangsu Province and Tangkou of Shandong Province, and gene may be involved in the -induced cytoplasmic incompatibility among three natural populations of .
研究中国东部三个自然种群致倦库蚊中与沃尔巴克氏体诱导的细胞质不亲和性相关的基因，以便为基于沃尔巴克氏体的蚊媒疾病防控措施的制定提供见解。
使用正反交试验检测从江苏省南京和无锡以及山东省唐口采集的三个致倦库蚊自然种群之间的细胞质不亲和性。采用聚合酶链反应（PCR）检测致倦库蚊中的沃尔巴克氏体感染情况，并使用荧光定量实时聚合酶链反应（qPCR）检测wsp和groEL基因的表达量。
江苏省南京和无锡采集的致倦库蚊自然种群之间存在双向亲和性（P = 0.57和0.15，两个P值均> 0.05），而山东省唐口和江苏省无锡采集的致倦库蚊自然种群之间以及江苏省南京和山东省唐口采集的致倦库蚊自然种群之间存在双向不亲和性（P = 63.81和43.51，两个P值均< 0.01）。在所有三个致倦库蚊自然种群中均扩增出wsp基因，qPCR检测发现三个致倦库蚊自然种群之间wsp基因表达无显著差异（P = 2.15，P > 0.05）。此外，山东省唐口和江苏省南京采集的致倦库蚊自然种群之间以及山东省唐口和江苏省无锡采集的致倦库蚊自然种群之间groEL基因表达无显著差异（P = 8.42，P < 0.05）；然而，江苏省南京和无锡采集的致倦库蚊自然种群之间groEL基因表达存在显著差异（P = 0.40，P > 0.05）。
在江苏省南京和无锡以及山东省唐口采集的致倦库蚊自然种群中检测到不同的沃尔巴克氏体数量，wsp基因可能参与了三个致倦库蚊自然种群中沃尔巴克氏体诱导的细胞质不亲和性。
