Food Biotechnology Program, KFRI School, Korea University of Science and Technology, Daejeon, Korea.
Division of Functional Food Research, Korea Food Research Institute, Wanju-gun, Korea.
J Med Food. 2020 Dec;23(12):1287-1295. doi: 10.1089/jmf.2020.4767. Epub 2020 Nov 12.
Allergic disorders, including atopic dermatitis (AD), are closely linked to the activation of type 2 helper T (Th2) cells. The aim of this study was to investigate the possibility of using fructus extract (RMFE) for AD treatment in the AD-like mouse model induced by treatment with trimellitic anhydride (TMA). Oral treatment of RMFE reduced the increase in ear thickness and suppressed inflammatory cytokine expression (interleukin [IL]-1 and tumor necrosis factor [TNF]-) and Th2-associated immune responses (immunoglobulin [Ig] E and IL-4) in mouse ears. Furthermore, messenger RNA (mRNA) expression levels such as IL-4, IL-5, and IL-13, in draining lymph nodes were decreased by RMFE. Furthermore, we found that RMFE increased the level of heme oxygenase-1 (HO-1) through ERK and p38 pathways, reducing IL-2 production and CD4 T cell proliferation, and inhibited STAT6 phosphorylation. Therefore, this study suggested that RMFE could be an effective treatment of AD induced by Th2-mediated immune responses by suppressing proliferation of CD4 T cells via increased HO-1.
变应性疾病，包括特应性皮炎（AD），与辅助性 T 细胞 2 型（Th2）的激活密切相关。本研究旨在探讨使用提取物（RMFE）治疗由三甲基戊烯二酸酐（TMA）诱导的 AD 样小鼠模型的可能性。RMFE 的口服治疗可减少耳厚度的增加，并抑制炎性细胞因子（白细胞介素[IL]-1 和肿瘤坏死因子[TNF]-）和 Th2 相关免疫反应（免疫球蛋白[Ig] E 和 IL-4）在小鼠耳朵中的表达。此外，RMFE 还降低了引流淋巴结中 IL-4、IL-5 和 IL-13 等信使 RNA（mRNA）的表达水平。此外，我们发现 RMFE 通过 ERK 和 p38 途径增加血红素加氧酶-1（HO-1）的水平，减少 IL-2 的产生和 CD4 T 细胞的增殖，并抑制 STAT6 的磷酸化。因此，本研究表明，RMFE 可通过增加 HO-1 抑制 CD4 T 细胞的增殖，从而有效治疗由 Th2 介导的免疫反应引起的 AD。
