Choi Dae Woon, Jung Sun Young, Kim Gun-Dong, Lee So-Young, Shin Hee Soon
Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea.
Division of Functional Food Research, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon 55365, Korea.
Antioxidants (Basel). 2021 Jul 13;10(7):1120. doi: 10.3390/antiox10071120.
Allergic diseases, including atopic dermatitis (AD), induce type 2 helper T (Th2) cell-dominant immune responses. Miquelianin (quercetin 3--glucuronide, MQL) is an active compound in fructus extract with anti-allergic properties. Here, we investigate the anti-allergic effects of MQL in an ovalbumin (OVA)-induced Th2-dominant mouse model and the associated mechanisms. Oral MQL suppressed cytokine and IL-2 production and proliferation of Th2 cells and upregulated heme oxygenase-1 (HO-1) in splenocytes. Ex vivo MQL suppressed Th1- and Th2-related immune responses by inhibiting CD4 T cell proliferation, and upregulated HO-1 in CD4 T cells by activating C-Raf-ERK1/2-Nrf2 pathway via induction of reactive oxygen species generation. In a trimellitic anhydride-induced AD-like mouse model, both topical and oral MQL ameliorated AD symptoms by suppressing Th2 immune responses. Our results suggest that MQL is a potential therapeutic agent for CD4 T cell-mediated diseases, including allergic diseases.
包括特应性皮炎（AD）在内的过敏性疾病会引发2型辅助性T（Th2）细胞主导的免疫反应。密屈菜素（槲皮素3-O-葡萄糖醛酸苷，MQL）是果实提取物中的一种具有抗过敏特性的活性化合物。在此，我们研究了MQL在卵清蛋白（OVA）诱导的Th2主导的小鼠模型中的抗过敏作用及其相关机制。口服MQL可抑制细胞因子和IL-2的产生以及Th2细胞的增殖，并上调脾细胞中的血红素加氧酶-1（HO-1）。体外实验中，MQL通过抑制CD4 T细胞增殖来抑制Th1和Th2相关的免疫反应，并通过诱导活性氧生成激活C-Raf-ERK1/2-Nrf2途径，从而上调CD4 T细胞中的HO-1。在偏苯三酸酐诱导的类AD小鼠模型中，局部和口服MQL均通过抑制Th2免疫反应改善了AD症状。我们的结果表明，MQL是一种用于治疗包括过敏性疾病在内的CD4 T细胞介导疾病的潜在治疗剂。
