Aumüller G, Fuhrmann W, Krause W
Department of Anatomy and Cell Biology, Philipps-Universität, Marburg, Federal Republic of Germany.
Andrologia. 1987 Jan-Feb;19(1):9-17. doi: 10.1111/j.1439-0272.1987.tb01849.x.
Testicular biopsies from two brothers with pathologic spermatograms revealed a spermatogenetic arrest at early spermatid maturation. No sign of acrosome or sperm tail formation was found in spermatids. Using a polyclonal antibody against vimentin, Sertoli cells appeared in the normal shape and distribution pattern. At the ultrastructural level no significant pathologic alterations of Sertoli cells were visible. A monoclonal antibody against tubulin gave a diffuse perinuclear reaction in spermatogonia, spermatocytes and spermatids. Some tubulin-immunoreactive material was also present in the apical portions of the Sertoli cells. Ultrastructural studies of spermatids revealed a complete absence of the centrioles and axonemal structures in early spermatids. Acrosome formation was inhibited at the early Golgi stage. The numerous spermatids present within germinal epithelium contained an abundance of elongate mitochondria and membrane profiles surrounding the nucleus. The ultrastructural findings indicate a maturation stop of spermatids at a very early stage with complete inhibition of acrosome and sperm tail formation. The underlying mechanism could be a lack of specific structural proteins.
对两名精子图谱异常的兄弟进行睾丸活检，结果显示精子发生在早期精子细胞成熟阶段停滞。在精子细胞中未发现顶体或精子尾部形成的迹象。使用抗波形蛋白的多克隆抗体，支持细胞呈现正常的形态和分布模式。在超微结构水平上，未观察到支持细胞有明显的病理改变。抗微管蛋白的单克隆抗体在精原细胞、精母细胞和精子细胞中产生弥漫性的核周反应。在支持细胞的顶端部分也存在一些微管蛋白免疫反应性物质。对精子细胞的超微结构研究显示，早期精子细胞中完全没有中心粒和轴丝结构。顶体形成在高尔基体早期受到抑制。生精上皮内大量的精子细胞含有丰富的细长线粒体和围绕细胞核的膜结构。超微结构研究结果表明，精子细胞在非常早期就停止成熟，顶体和精子尾部形成完全受到抑制。潜在机制可能是缺乏特定的结构蛋白。
